The relationship between dlx and gad1 expression indicates highly conserved genetic pathways in the zebrafish forebrain.
The relationship between dlx and gad1 expression indicates highly conserved genetic pathways in the zebrafish forebrain.
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DLX和GAD1表达之间的关系表明斑马鱼前脑中高度保守的遗传途径。
DOI：
10.1002/dvdy.22365
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发表时间：
2010-08
影响因子：
2.5
通讯作者：
Ekker, Marc
中科院分区：
文献类型：
--
作者：
MacDonald, Ryan B.;Debiais-Thibaud, Melanie;Talbot, Jared Coffin;Ekker, Marc
Dlx基因编码对脊椎动物前脑发育重要的转录因子家族。这些基因在小鼠端脑发育过程中具有非常相似的表达结构域，并在GABA能中间神经元分化中发挥作用。我们利用三重荧光原位杂交技术研究了斑马鱼端脑和间脑中dlx和gad 1基因的相对表达区域。我们还产生了转基因斑马鱼的调控元件从斑马鱼dlx 1a/2a基因座。斑马鱼dlx调控元件重现了dlx在前脑的表达，并模拟了dlx基因表达与gad 1之间的关系。最后，我们发现，一个假定的增强子位于下游的dlx 2b也可以激活报告基因的表达，在组织特异性的方式类似于内源性dlx 2b的表达。我们的研究结果表明，dlx基因的调控进化保守的遗传途径，并可能在GABA能中间神经元的分化在斑马鱼前脑中发挥作用。
The Dlx genes encode a family of transcription factors important to the development of the vertebrate forebrain. These genes have very similar expression domains during the development of the telencephalon in mouse and play a role in GABAergic interneuron differentiation. We have used triple fluorescent in situ hybridization to study the relative expression domains of the dlx and gad1 genes in the zebrafish telencephalon and diencephalon. We also generated transgenic zebrafish with regulatory elements from the zebrafish dlx1a/2a locus. The zebrafish dlx regulatory elements recapitulated dlx expression in the forebrain and mimicked the relationship between the expression of the dlx genes and gad1. Finally, we show that a putative enhancer located downstream of dlx2b can also activate reporter gene expression in a tissue specific manner similar to endogenous dlx2b expression. Our results indicate the dlx genes are regulated by an evolutionarily conserved genetic pathway and may play a role in GABAergic interneuron differentiation in the zebrafish forebrain.
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影响因子：
5.3
作者：
Ghanem, Noel;Yu, Man;Ekker, Marc
通讯作者：
Ekker, Marc
影响因子：
2.5
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
通讯作者：
SCHILLING, TF
影响因子：
7
作者：
Ghanem, N;Jarinova, O;Ekker, M
通讯作者：
Ekker, M
DOI：
10.1016/s0165-3806(02)00583-7
发表时间：
2003-01-13
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
Mueller, T;Wullimann, MF
通讯作者：
Wullimann, MF
影响因子：
3.7
作者：
Stühmer, T;Puelles, L;Rubenstein, JLR
通讯作者：
Rubenstein, JLR