Exhaled volatile organic compounds in patients with non-small cell lung cancer: cross sectional and nested short-term follow-up study.
Exhaled volatile organic compounds in patients with non-small cell lung cancer: cross sectional and nested short-term follow-up study.
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非小细胞肺癌患者中呼出的挥发性有机化合物：横断面和嵌套的短期随访研究。
DOI：
10.1186/1465-9921-6-71
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发表时间：
2005-07-14
影响因子：
5.8
通讯作者：
Mutti, A
中科院分区：
文献类型：
--
作者：
Poli, D;Carbognani, P;Corradi, M;Goldoni, M;Acampa, O;Balbi, B;Bianchi, L;Rusca, M;Mutti, A
非侵入性的诊断策略，旨在确定肺癌的生物标志物是非常感兴趣的早期癌症检测。本研究的目的是建立一种新的方法来识别和定量非小细胞肺癌（NSCLC）患者呼出气体中的挥发性有机化合物（VOCs），通过与健康吸烟者和非吸烟者以及慢性阻塞性肺疾病患者的水平进行比较。在NSCLC患者接受肺手术后三周重复VOC收集和分析。将受试者的呼吸收集在Teflon®灯泡中，该灯泡捕获单次缓慢肺活量的最后部分。使用固相微萃取技术浓缩本研究选择的13种挥发性有机化合物，随后通过气相色谱/质谱法进行分析。所选VOC的水平范围从苯乙烯的10-12 M到异戊二烯的10-9 M。没有一种VOC单独区分研究组，因此不可能将一种单一的化合物鉴定为特定的肺癌生物标志物。然而，多项逻辑回归分析显示VOC谱可以正确分类约80%的病例。只有异戊二烯和癸烷水平在手术后显著降低。由于13种挥发性有机化合物的组合可以正确地将病例分组，再加上传统的诊断方法，挥发性有机化合物分析可以用作肺癌早期诊断的补充测试。根据我们的短期巢式研究结果，不能推荐其在手术患者随访中的可能用途。
Non-invasive diagnostic strategies aimed at identifying biomarkers of lung cancer are of great interest for early cancer detection. The aim of this study was to set up a new method for identifying and quantifying volatile organic compounds (VOCs) in exhaled air of patients with non-small cells lung cancer (NSCLC), by comparing the levels with those obtained from healthy smokers and non-smokers, and patients with chronic obstructive pulmonary disease. The VOC collection and analyses were repeated three weeks after the NSCLC patients underwent lung surgery. The subjects' breath was collected in a Teflon® bulb that traps the last portion of single slow vital capacity. The 13 VOCs selected for this study were concentrated using a solid phase microextraction technique and subsequently analysed by means of gas cromatography/mass spectrometry. The levels of the selected VOCs ranged from 10-12 M for styrene to 10-9 M for isoprene. None of VOCs alone discriminated the study groups, and so it was not possible to identify one single chemical compound as a specific lung cancer biomarker. However, multinomial logistic regression analysis showed that VOC profile can correctly classify about 80 % of cases. Only isoprene and decane levels significantly decreased after surgery. As the combination of the 13 VOCs allowed the correct classification of the cases into groups, together with conventional diagnostic approaches, VOC analysis could be used as a complementary test for the early diagnosis of lung cancer. Its possible use in the follow-up of operated patients cannot be recommended on the basis of the results of our short-term nested study.
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影响因子：
168.9
作者：
Phillips, M;Gleeson, K;McVay, WP
通讯作者：
McVay, WP
影响因子：
6.3
作者：
Roback, PJ;Askins, RA
通讯作者：
Askins, RA
影响因子：
5
作者：
Tsai, JH;Chiang, HL;Yang, CY
通讯作者：
Yang, CY
影响因子：
5
作者：
Edwards, RD;Jurvelin, J;Jantunen, M
通讯作者：
Jantunen, M
DOI：
10.1016/0378-4347(95)00207-y
发表时间：
1995-10-06
影响因子：
3
作者：
JONES, AW;LAGESSON, V;TAGESSON, C
通讯作者：
TAGESSON, C