Propolis Ameliorates Alcohol-Induced Depressive Symptoms in C57BL/6J Mice by Regulating Intestinal Mucosal Barrier Function and Inflammatory Reaction.
Propolis Ameliorates Alcohol-Induced Depressive Symptoms in C57BL/6J Mice by Regulating Intestinal Mucosal Barrier Function and Inflammatory Reaction.
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蜂胶通过调节肠粘膜屏障功能和炎症反应改善 C57BL/6J 小鼠酒精引起的抑郁症状
DOI：
10.3390/nu14061213
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发表时间：
2022-03-12
期刊：
影响因子：
5.9
通讯作者：
Liang H
中科院分区：
文献类型：
--
作者：
Wang P;Guo P;Wang Y;Teng X;Zhang H;Sun L;Xue M;Liang H
越来越多的证据表明，脑肠轴作为许多中枢神经系统疾病的重要范例发挥着关键作用。近年来的研究表明，黄芪具有明显的神经保护特性和调节肠道菌群植物群的功能，提示在该轴调节的两个末端具有潜在的关键作用。然而，目前还没有明确的证据证实酒精对酒精引起的抑郁症的影响。在这里，我们用C57 BL/6 J小鼠建立了酒精性抑郁症模型，并通过行为测试证明了用甘草酸治疗可以预防酒精诱导的抑郁症状。此外，甘草酸还能减轻酒精诱导的抑郁症小鼠海马区神经细胞的损伤，恢复血清中脑源性神经营养因子（BDNF）和多巴胺（DA）的水平。病理学和生物素示踪试验表明，黄芪能修复酒精引起的肠漏。此外，顺铂处理增加了肠细胞间紧密连接（TJ）结构蛋白Claudin-1、Occludin和Claudin occludens-1（ZO-1）的表达水平，以及与肠通透性密切相关的肝激酶B1/AMP-活化蛋白激酶（LKB 1/AMPK）信号通路的活化状态。此外，酒精性抑郁症可以降低促炎、脂多糖（LPS）和脂肪酸结合蛋白2（FABP 2）的水平，这表明炎症反应在酒精性抑郁症中的重要性。总的来说，我们的研究结果表明，降血糖通过改善脑肠道功能障碍对酒精引起的抑郁症状产生了改善作用。
Accumulating evidence points to a critical role of the brain gut axis as an important paradigm for many central nervous system diseases. Recent studies suggest that propolis has obvious neuroprotective properties and functionality in regulating intestinal bacteria flora, hinting at a potential key effect at both terminals of this axis regulation. However, currently no clear evidence confirms the effects of propolis on alcohol-induced depression. Here, we establish an alcoholic depression model with C57BL/6J mice and demonstrate that treatment with propolis protects against alcohol-induced depressive symptoms by behavioral tests. In addition, propolis attenuates the injury of nerve cells in the hippocampal region and restores the serum levels of brain-derived neurotrophic factor (BDNF) and dopamine (DA) in mice with alcohol-induced depression. Pathology and biotin tracer assays show that propolis repairs the intestinal leakage caused by alcohol. Additionally, propolis treatment increases the expression levels of intestinal intercellular tight junctions’ (TJs’) structural proteins Claudin-1, Occludin and zona occludens-1 (ZO-1), as well as the activation state of the liver kinase B1/AMP-activated protein kinase (LKB1/AMPK) signaling pathway, which is closely related to the intestinal permeability. Furthermore, propolis can reduce the levels of pro-inflammatory, lipopolysaccharide (LPS) and fatty-acid-binding protein 2 (FABP2), suggesting the significance of the inflammatory response in alcoholic depression. Collectively, our findings indicate that propolis exerted an improving effect on alcohol-induced depressive symptoms by ameliorating brain gut dysfunction.
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影响因子：
6.6
作者：
Brunoni, Andre R.;Supasitthumrong, Thitiporn;Maes, Michael
通讯作者：
Maes, Michael
DOI：
10.1001/jama.2017.3826
发表时间：
2017-04-18
期刊：
JAMA
影响因子：
--
作者：
Friedrich, M J
通讯作者：
Friedrich, M J
影响因子：
8.8
作者：
Leclercq, Sophie;Le Roy, Tiphaine;Delzenne, Nathalie M.
通讯作者：
Delzenne, Nathalie M.
DOI：
10.1152/ajpgi.00236.2013
发表时间：
2014-04-01
影响因子：
4.5
作者：
Elamin, Elhaseen;Masclee, Ad;Jonkers, Daisy
通讯作者：
Jonkers, Daisy
DOI：
10.1152/ajpcell.2000.279.1.c21
发表时间：
2000-07-01
影响因子：
5.5
作者：
Kevil, CG;Oshima, T;Alexander, JS
通讯作者：
Alexander, JS