Successful immunization against gastric infection with Helicobacter species: use of a cholera toxin B-subunit-whole-cell vaccine
Successful immunization against gastric infection with Helicobacter species: use of a cholera toxin B-subunit-whole-cell vaccine
复制标题
成功免疫螺杆菌属胃部感染：使用霍乱毒素 B 亚基全细胞疫苗
作者：
Adrian K. C. Lee;Minhui Chen
在之前的研究中，我们发现用猫螺杆菌超声处理和佐剂霍乱毒素（CT；10微克）对小鼠进行免疫接种可以保护动物免受活猫螺杆菌的攻击。本研究的目的是确定低剂量 CT 或其无毒 B 亚基 (CTB) 作为螺杆菌口服疫苗的佐剂是否有效。在用猫嗜血杆菌抗原加0.5微克CT和10微克CTB的组合免疫的动物(96%)、用猫嗜血杆菌抗原加0.5微克CT(95%)、以及用猫嗜血杆菌抗原加10微克CTB(83%)免疫的动物中，获得了针对活猫嗜血杆菌攻击的显着保护。在单独用猫嗜血杆菌抗原或单独用佐剂CTB和CT免疫的小鼠中没有发现保护作用。接受幽门螺杆菌抗原加 CT（10 微克）免疫的小鼠中有 26% 能够免受猫幽门螺杆菌攻击，证实了该模型在预测人类免疫效果方面的价值。使用无毒佐剂 CTB 可以诱导免疫的观察结果为幽门螺杆菌疫苗的人体研究开辟了道路，并且是在有效预防具有世界重大意义的胃十二指肠疾病的策略道路上又向前迈出了一步。
In previous studies we found that immunizing mice with a sonicate of Helicobacter felis and adjuvant cholera toxin (CT; 10 micrograms) protected the animals against challenge with viable H. felis. The aim of this study was to determine whether a low dose of CT or its nontoxic B subunit (CTB) was effective as an adjuvant in Helicobacter oral vaccines. Significant protection against viable H. felis challenge was achieved in the animals immunized with H. felis antigen plus the combination of 0.5 microgram of CT and 10 micrograms of CTB (96%), with H. felis antigen plus 0.5 microgram of CT (95%), and with H. felis antigen plus 10 micrograms of CTB (83%). No protective effect was found in the mice immunized with either H. felis antigen alone or adjuvant CTB and CT alone. Twenty-six percent of mice immunized with Helicobacter pylori antigen plus CT (10 micrograms) were protected against H. felis challenge, confirming the value of the model in predicting effects of immunization in humans. The observation that immunity can be induced with a nontoxic adjuvant CTB opens the way for human studies with H. pylori vaccines and is a further step along the road to effective strategies of prevention of gastroduodenal diseases of major world significance.
影响因子：
5.5
作者：
CZINN, SJ;CAI, A;NEDRUD, JG
通讯作者：
NEDRUD, JG
影响因子：
158.5
作者：
PARSONNET, J;FRIEDMAN, GD;SIBLEY, RK
通讯作者：
SIBLEY, RK