A monolithic lipase reactor for biodiesel production by transesterification of triacylglycerides into fatty acid methyl esters.
A monolithic lipase reactor for biodiesel production by transesterification of triacylglycerides into fatty acid methyl esters.
复制标题
DOI：
10.1002/bit.23326
复制
发表时间：
2012-02
影响因子：
3.8
通讯作者：
Frechet, Jean M. J.
中科院分区：
文献类型：
--
作者：
Urban, Jiri;Svec, Frantisek;Frechet, Jean M. J.
关键词：
制备了一种固定化脂肪酶的酶反应器，用于催化三酰甘油酯转酯为生物柴油常用的脂肪酸甲酯。实验过程的设计用于优化整体式反应器，变量包括通过甲基丙烯酸烷基酯单体中的烃链长度的变化来控制整体式的表面极性，用于活化整体式的1-乙烯基-4，4-二甲基氮内酯的接枝时间，以及用于固定化猪脂肪酶的时间。最佳条件涉及使用的聚（甲基丙烯酸硬脂酯-共-乙烯二甲基丙烯酸酯）整料，接枝第一与vinylazlactone，然后用脂肪酶处理2小时进行固定化的酶。用于三丁酸甘油酯的酯交换的最佳条件包括37°C的温度和底物在生物反应器中的10分钟停留时间。即使在泵入相当于1，000反应器体积的底物溶液后，反应器也没有失去活性。该酶反应器还用于从大豆油到脂肪酸甲酯的三酰甘油酯的酯交换，从而证明了该反应器生产生物柴油的能力。
An enzymatic reactor with lipase immobilized on a monolithic polymer support has been prepared and used to catalyze the transesterification of triacylglycerides into the fatty acid methyl esters commonly used for biodiesel. A design of experiments procedure was used to optimize the monolithic reactor with variables including control of the surface polarity of the monolith via variations in the length of the hydrocarbon chain in alkyl methacrylate monomer, time of grafting of 1-vinyl-4,4-dimethylazlactone used to activate the monolith, and time used for the immobilization of porcine lipase. Optimal conditions involved the use of a poly(stearyl methacrylate-co-ethylene dimethacrylate) monolith, grafted first with vinylazlactone, then treated with lipase for 2 h to carry out the immobilization of the enzyme. Best conditions for the transesterification of glyceryl tributyrate included a temperature of 37°C and a 10 min residence time of the substrate in the bioreactor. The reactor did not lose its activity even after pumping through it a solution of substrate equaling 1,000 reactor volumes. This enzymatic reactor was also used for the transesterification of triacylglycerides from soybean oil to fatty acid methyl esters thus demonstrating the ability of the reactor to produce biodiesel.
登录
查看更多内容
影响因子：
5.5
作者：
Rohr, T;Hilder, EF;Fréchet, JMJ
通讯作者：
Fréchet, JMJ
影响因子：
5.1
作者：
Drtina, GJ;Haddad, LC;Heilmann, SM
通讯作者：
Heilmann, SM
影响因子：
6.2
作者：
Reddy, SM;Vadgama, P
通讯作者：
Vadgama, P
影响因子：
4.4
作者：
Halim, Siti Fatimah Abdul;Kamaruddin, Azlina Harun
通讯作者：
Kamaruddin, Azlina Harun
影响因子：
4.1
作者：
Bartolini, M;Cavrini, V;Andrisano, V
通讯作者：
Andrisano, V