Establishment of a stable transfection system for genetic manipulation of Babesia gibsoni.
Establishment of a stable transfection system for genetic manipulation of Babesia gibsoni.
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DOI：
10.1186/s13071-018-2853-1
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发表时间：
2018-04-23
影响因子：
3.2
通讯作者：
Xuan X
中科院分区：
文献类型：
--
作者：
Liu M;Adjou Moumouni PF;Asada M;Hakimi H;Masatani T;Vudriko P;Lee SH;Kawazu SI;Yamagishi J;Xuan X
在许多原生动物寄生虫中，已经报道过基因操纵技术，如转染法。在巴贝斯虫中，只有牛巴贝斯虫才建立了稳定的转导系统。我们最近报道了吉氏巴贝斯虫的瞬时转移系统和启动子候选的选择。为了更好地控制巴贝斯虫病，迫切需要建立稳定的吉氏杆菌基因表达系统，以提高我们对犬巴贝斯虫病基本生物学的了解。荧光显微镜下观察到表达GFP的寄生虫最早在用药后2周，并在无药物压力的情况下持续表达GFP 3个月以上。经聚合酶链式反应、测序和Southern印迹分析证实基因组整合。我们首次成功地建立了吉氏芽胞杆菌稳定的转导系统。这一发现将有助于利用基因操作对巴贝斯虫基因组进行功能分析，并将为发展硬虫-巴贝斯虫和宿主-巴贝斯虫感染模型奠定基础。本文的在线版本(10.1186/s13071-0182853-1)包含向授权用户提供的补充材料。
Genetic manipulation techniques, such as transfection, have been previously reported in many protozoan parasites. In Babesia, stable transfection systems have only been established for bovine Babesia parasites. We recently reported a transient transfection system and the selection of promoter candidates for Babesia gibsoni. The establishment of a stable transfection system for B. gibsoni is considered to be urgent to improve our understanding of the basic biology of canine Babesia parasites for a better control of babesiosis. GFP-expressing parasites were observed by fluorescence microscopy as early as two weeks after drug selection, and consistently expressed GFP for more than 3 months without drug pressure. Genome integration was confirmed by PCR, sequencing and Southern blot analysis. We present the first successful establishment of a stable transfection system for B. gibsoni. This finding will facilitate functional analysis of Babesia genomes using genetic manipulation and will serve as a foundation for the development of tick-Babesia and host-Babesia infection models. The online version of this article (10.1186/s13071-018-2853-1) contains supplementary material, which is available to authorized users.
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影响因子：
1.5
作者：
Ganesan, Suresh M.;Morrisey, Joanne M.;Ke, Hangjun;Painter, Heather J.;Laroiya, Kamal;Phillips, Margaret A.;Rathod, Pradipsinh K.;Mather, Michael W.;Vaidya, Akhil B.
通讯作者：
Vaidya, Akhil B.
建立一种新型的tick虫实验感染模型。
影响因子：
4.6
作者：
Maeda H;Hatta T;Alim MA;Tsubokawa D;Mikami F;Matsubayashi M;Miyoshi T;Umemiya-Shirafuji R;Kawazu SI;Igarashi I;Mochizuki M;Tsuji N;Tanaka T
通讯作者：
Tanaka T
影响因子：
2.6
作者：
Suarez, Carlos E.;Noh, Susan
通讯作者：
Noh, Susan
影响因子：
4
作者：
Suarez, Carlos E.;McElwain, Terry F.
通讯作者：
McElwain, Terry F.
影响因子：
64.8
作者：
Vinayak S;Pawlowic MC;Sateriale A;Brooks CF;Studstill CJ;Bar-Peled Y;Cipriano MJ;Striepen B
通讯作者：
Striepen B