In vivo priming of human normal neutrophils by granulocyte‐macrophage colony stimulating factor: effect on the production of platelet activating factor
In vivo priming of human normal neutrophils by granulocyte‐macrophage colony stimulating factor: effect on the production of platelet activating factor
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粒细胞巨噬细胞集落刺激因子体内启动人正常中性粒细胞：对血小板激活因子产生的影响
DOI：
10.1111/j.1365-2141.1990.tb04345.x
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发表时间：
1990
影响因子：
6.5
通讯作者：
F. Bussolino
中科院分区：
文献类型：
--
作者：
M. Aglietta;C. Monzeglio;Franco Apra;C. Mossetti;A. Stern;G. Giribaldi;F. Bussolino
概括。粒细胞巨噬细胞集落刺激因子 (GM-CSF)（重组、哺乳动物、糖基化、Sandoz、先灵葆雅；每 12 小时 4 μg/kg，连续 3 天，皮下注射）对中性粒细胞产生血小板激活因子（PAF、1-O-烷基-2-乙酰基-sn 甘油-3 磷酸胆碱）的影响 在五名造血正常的癌症患者中进行了研究。在治疗前、治疗期间和治疗停止后 3 天评估外周血计数、PAF 产生和溶血 PAF：中性粒细胞中的乙酰转移酶 (EC 2.3.1.67) (AT) 活性。 GM-CSF 诱导循环中性粒细胞数量增加三倍。治疗期间获得的中性粒细胞响应各种刺激（N-甲酰基-甲硫氨酰-亮氨酰-苯丙氨酸、肿瘤坏死因子-α、C3b调理的面包酵母孢子的吞噬作用）产生的PAF大约是治疗前的两倍。 PAF 合成和释放的增加伴随着 AT 活性增加 2-3 倍。此外，较低浓度的刺激足以诱导 GM-CSF 治疗期间获得的中性粒细胞合成 PAF。治疗停止三天后，刺激诱导的 PAF 产生已恢复至基线水平。由于 GM-CSF 引起 Arneth 评分明显向左移动，PAF 释放增加可能是由于年轻粒细胞的存在。然而，实验排除了这一点，实验表明，在体外用 GM-CSF 引发的正常中性粒细胞在受到相同刺激时会产生更多的 PAF。 GM-CSF 治疗的这种作用的潜在相关性在于 PAF 在炎症反应中的关键作用及其对某些免疫反应（包括迟发型超敏反应）和内毒素休克的干预。最后，中性粒细胞产生的 PAF 增加可能解释了高剂量 GM-CSF 治疗期间观察到的一些毒性。
Summary. The effect of granulocyte‐macrophage colony stimulating factor (GM‐CSF) (recombinant, mammalian, glycosylated, Sandoz, Schering Plough; 4 μg/kg every 12 h for 3 d, s.c.) on platelet activating factor (PAF, 1‐O‐alkyl‐2‐acetyl‐sn glycero‐3 phosphorylcholine) production from neutrophils was studied in five cancer patients with normal haemopoiesis. Peripheral blood counts, PAF production and lyso‐PAF: acetyl transferase (EC 2.3.1.67) (AT) activity in neutrophils were evaluated before treatment, during treatment and 3 d after treatment had been discontinued. GM‐CSF induced a three‐fold increase in the number of circulating neutrophils. Neutrophils obtained during treatment produced about twice as much PAF than before treatment in response to a variety of stimuli (N‐formyl‐methionyl‐leucyl‐phenylalanine, tumour necrosis factor‐alpha, phagocytosis of baker's yeast spores opsonized with C3b). This increased PAF synthesis and release is concomitant with a 2‐3‐fold increase in AT activity. Moreover, lower concentrations of stimuli are sufficient to induce PAF synthesis from neutrophils obtained during GM‐CSF treatment. Three days after treatment had been discontinued, stimulus induced PAF production had returned to baseline levels. Since GM‐CSF induces a marked shift to the left in the Arneth score, the increased PAF release might have been due to the presence of younger granulo‐cytes. This was, however, ruled out by experiments showing that normal neutrophils primed in vitro with GM‐CSF produce more PAF when challenged with the same stimuli. The potential relevance of this effect of GM‐CSF treatment lies on the crucial role of PAF in inflammatory reactions and its intervention in some immune reactions, including delayed hypersensitivity, and in endotoxic shock. Lastly, increased PAF production from neutrophils may explain some toxicities observed during treatment with high doses of GM‐CSF.
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人类中性粒细胞和嗜酸性粒细胞中血小板激活因子（1-烷基-2-乙酰基-sn-甘油-3-磷酸胆碱）代谢酶的活性以及钙离子载体的作用。
DOI：
10.1016/0006-291x(82)90928-7
发表时间：
1982
影响因子：
3.1
作者：
Lee,TC;Malone,B;Wasserman,SI;Fitzgerald,V;Snyder,F
通讯作者：
Snyder,F
用 IL-1-β、肿瘤坏死因子或 IFN-γ 刺激的人单核细胞合成双相血小板激活因子。
DOI：
--
发表时间：
1988
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Valone,FH;Epstein,LB
通讯作者：
Epstein,LB
用肿瘤坏死因子或白细胞介素 1 α 处理的人血管内皮细胞合成和释放血小板激活因子。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Bussolino,F;Camussi,G;Baglioni,C
通讯作者：
Baglioni,C
重组人粒细胞-巨噬细胞集落刺激因子在淋巴恶性肿瘤自体骨髓移植中的应用。
影响因子：
20.3
作者：
Nemunaitis,J;Singer,JW;Buckner,CD;Hill,R;Storb,R;Thomas,ED;Appelbaum,FR
通讯作者：
Appelbaum,FR
重组人粒细胞巨噬细胞集落刺激因子对难治性癌患者中性粒细胞活化的影响。
影响因子：
20.3
作者：
Kaplan,SS;Basford,RE;Wing,EJ;Shadduck,RK
通讯作者：
Shadduck,RK