Genomic surveillance of Salmonella spp. in the Philippines during 2013-2014.
Genomic surveillance of Salmonella spp. in the Philippines during 2013-2014.
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DOI：
10.1093/trstmh/trac080
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发表时间：
2022-12-02
影响因子：
2.2
通讯作者：
中科院分区：
文献类型：
--
作者：
在菲律宾，沙门氏菌的抗菌素耐药性（AMR）不断增加。本研究旨在通过全基因组测序技术（WGS）研究沙门氏菌的种群和AMR机制，并与实验室监测方法进行比较。对148株伤寒沙门氏菌进行了血清型、多位点序列型、AMR基因及菌株间亲缘关系的研究。伤寒）和65个非伤寒沙门氏菌（NTS）收集的抗菌素耐药性监测计划在2013-2014年。将基因型血清型和AMR预测与表型数据进行比较。S.伤寒是低，与氟喹诺酮类或超广谱β-内酰胺酶（ESBL）基因的敏感性降低相关的突变稀疏收购。相比之下，75%的NTS分离株对至少一种抗生素不敏感，其中一半以上携带与氟喹诺酮耐药相关的突变和/或基因。在5个基因组中检测到ESBL基因，这些基因组还携带其他AMR决定簇。S.伤寒主要是由可能的地方性基因型3.0，这引起了假定的本地爆发。NTS的主要分支是全球流行的S。肠杆菌ST 11和S.鼠伤寒沙门氏菌双相变异体（I，4，[5]，12：i：-）ST 34.我们提供了来自菲律宾的沙门氏菌的第一个基因组特征和WGS实用程序用于持续监测的证据。
Increasing antimicrobial resistance (AMR) in Salmonella has been observed in the Philippines. We aimed to characterise the population and AMR mechanisms of Salmonella with whole genome sequencing (WGS) and compare it with laboratory surveillance methods. The serotype, multilocus sequence type, AMR genes and relatedness between isolates were determined from the genomes of 148 Salmonella Typhi (S. Typhi) and 65 non-typhoidal Salmonella (NTS) collected by the Antimicrobial Resistance Surveillance Program during 2013–2014. Genotypic serotypes and AMR prediction were compared with phenotypic data. AMR rates in S. Typhi were low, with sparse acquisition of mutations associated with reduced susceptibility to fluoroquinolones or extended-spectrum beta-lactamases (ESBL) genes. By contrast, 75% of NTS isolates were insusceptible to at least one antimicrobial, with more than half carrying mutations and/or genes linked to fluoroquinolone resistance. ESBL genes were detected in five genomes, which also carried other AMR determinants. The population of S. Typhi was dominated by likely endemic genotype 3.0, which caused a putative local outbreak. The main NTS clades were global epidemic S. Enteritidis ST11 and S. Typhimurium monophasic variant (I,4,[5],12: i: -) ST34. We provide the first genomic characterisation of Salmonella from the Philippines and evidence of WGS utility for ongoing surveillance.
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在病原体观察中，鼠伤寒沙门氏菌抗菌耐药性和监测的基因组预测的全球资源。
影响因子：
16.6
作者：
Argimón S;Yeats CA;Goater RJ;Abudahab K;Taylor B;Underwood A;Sánchez-Busó L;Wong VK;Dyson ZA;Nair S;Park SE;Marks F;Page AJ;Keane JA;Baker S;Holt KE;Dougan G;Aanensen DM
通讯作者：
Aanensen DM
影响因子：
30.8
作者：
Holt, Kathryn E.;Parkhill, Julian;Mazzoni, Camila J.;Roumagnac, Philippe;Weill, Francois-Xavier;Goodhead, Ian;Rance, Richard;Baker, Stephen;Maskell, Duncan J.;Wain, John;Dolecek, Christiane;Achtman, Mark;Dougan, Gordon
通讯作者：
Dougan, Gordon
影响因子：
9.9
作者：
Franz, Eelco;Gras, Lapo Mughini;Dallman, Tim
通讯作者：
Dallman, Tim
影响因子：
9.4
作者：
Hendriksen, Rene S.;Leekitcharoenphon, Pimlapas;Hasman, Henrik
通讯作者：
Hasman, Henrik
影响因子：
6.4
作者：
Mather AE;Phuong TLT;Gao Y;Clare S;Mukhopadhyay S;Goulding DA;Hoang NTD;Tuyen HT;Lan NPH;Thompson CN;Trang NHT;Carrique-Mas J;Tue NT;Campbell JI;Rabaa MA;Thanh DP;Harcourt K;Hoa NT;Trung NV;Schultsz C;Perron GG;Coia JE;Brown DJ;Okoro C;Parkhill J;Thomson NR;Chau NVV;Thwaites GE;Maskell DJ;Dougan G;Kenney LJ;Baker S
通讯作者：
Baker S