Glycosylphosphatidylinositol Anchors from Galactomannan and GPI-Anchored Protein Are Synthesized by Distinct Pathways in Aspergillus fumigatus.
Glycosylphosphatidylinositol Anchors from Galactomannan and GPI-Anchored Protein Are Synthesized by Distinct Pathways in Aspergillus fumigatus.
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DOI：
10.3390/jof4010019
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发表时间：
2018-02-02
期刊：
影响因子：
--
通讯作者：
Fontaine T
中科院分区：
文献类型：
--
作者：
Li J;Mouyna I;Henry C;Moyrand F;Malosse C;Chamot-Rooke J;Janbon G;Latgé JP;Fontaine T
糖基磷脂酰肌醇（GPIs）是允许蛋白质暴露在质膜外层的脂质锚。在真菌中，许多GPI锚定蛋白（GPI-AP）参与细胞壁聚合物的重塑。GPIs在内质网中遵循特定的生物合成途径。在将蛋白质转移到GPI锚上后，发生脂质重塑以用神经酰胺取代二酰基甘油部分。除GPI-AP外，A.烟曲霉产生GPI锚定的多糖，半乳甘露聚糖（GM），其在真菌界中仍然是独特的。为了研究GPI途径在GM生物合成和细胞壁组织中的作用，进行了PER 1的缺失-编码GPI脂质重塑的第一步所需的磷脂酶。从GPI-AP分离的GPI-锚的生物化学表征显示PER 1缺陷突变体产生具有二酰基甘油的脂质锚。在Δ per 1突变体中GPI锚点上神经酰胺的缺失导致GPI-AP的错误定位和细胞壁碱不溶性组分的组成改变。另一方面，从Δ per 1突变体膜分离的GM具有与亲本菌株相同的神经酰胺部分，表明GM的GPI锚遵循独特的未知生物合成途径。
Glycosylphosphatidylinositols (GPIs) are lipid anchors allowing the exposure of proteins at the outer layer of the plasma membrane. In fungi, a number of GPI-anchored proteins (GPI-APs) are involved in the remodeling of the cell wall polymers. GPIs follow a specific biosynthetic pathway in the endoplasmic reticulum. After the transfer of the protein onto the GPI-anchor, a lipid remodeling occurs to substitute the diacylglycerol moiety by a ceramide. In addition to GPI-APs, A. fumigatus produces a GPI-anchored polysaccharide, the galactomannan (GM), that remains unique in the fungal kingdom. To investigate the role of the GPI pathway in the biosynthesis of the GM and cell wall organization, the deletion of PER1—coding for a phospholipase required for the first step of the GPI lipid remodeling—was undertaken. Biochemical characterization of the GPI-anchor isolated from GPI-APs showed that the PER1 deficient mutant produced a lipid anchor with a diacylglycerol. The absence of a ceramide on GPI-anchors in the Δper1 mutant led to a mislocation of GPI-APs and to an alteration of the composition of the cell wall alkali-insoluble fraction. On the other hand, the GM isolated from the Δper1 mutant membranes possesses a ceramide moiety as the parental strain, showing that GPI anchor of the GM follow a distinct unknown biosynthetic pathway.
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影响因子：
3.6
作者：
Komachi Y;Hatakeyama S;Motomatsu H;Futagami T;Kizjakina K;Sobrado P;Ekino K;Takegawa K;Goto M;Nomura Y;Oka T
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3
作者：
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3.4
作者：
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影响因子：
2.8
作者：
Bernard, M;Mouyna, I;Latgé, JP
通讯作者：
Latgé, JP
影响因子：
3.3
作者：
Bosson, Regine;Jaquenoud, Malika;Conzelmann, Andreas
通讯作者：
Conzelmann, Andreas