Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells.
Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells.
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DOI：
10.1016/j.xpro.2020.100068
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发表时间：
2020-09-18
期刊：
影响因子：
--
通讯作者：
Ryan AL
中科院分区：
文献类型：
--
作者：
Hor P;Ichida JK;Borok Z;Ryan AL
肺神经内分泌细胞（PNECs）是肺气道上皮内的感觉细胞。在这里，我们提供了一个从人类诱导多能干细胞（iPSCs）生成诱导PNECs （iPNECs）的详细方案。ipnec的细胞和分子特征与人类原发性pnec相似。原发的人类pnec极为罕见，仅占成人肺的1%。因此，患者特异性ipnec的自我更新来源有助于创建可重复的人类细胞模型，以研究以PNEC功能障碍为特征的肺部疾病。有关使用和执行本协议的完整详情，请参阅。从人ipsc气液界面培养中高效生成iPNEC提高iPNEC规格持续Notch抑制提高iPNEC规格iPNEC基因表达特征与原代人胎儿pnec高度一致肺神经内分泌细胞（pnec）是肺气道上皮内的感觉细胞。在这里，我们提供了一个从人类诱导多能干细胞（iPSCs）生成诱导PNECs （iPNECs）的详细方案。ipnec的细胞和分子特征与人类原发性pnec相似。原发的人类pnec极为罕见，仅占成人肺的1%。因此，患者特异性ipnec的自我更新来源有助于创建可重复的人类细胞模型，以研究以PNEC功能障碍为特征的肺部疾病。
Pulmonary neuroendocrine cells (PNECs) are sensory cells within the lung airway epithelia. Here, we provide a detailed protocol for generating induced PNECs (iPNECs) from human induced pluripotent stem cells (iPSCs). The cellular and molecular profile of iPNECs resembles primary human PNECs. Primary human PNECs are exceedingly rare, comprising only 1% of the adult lung. Therefore, a self-renewing source of patient-specific iPNECs facilitates the creation of reproducible human cellular models to study lung diseases characterized by PNEC dysfunction. For complete details on the use and execution of this protocol, please refer to. Efficient generation of iPNECs from human iPSCs Air-liquid-interface culture augments iPNEC specification Continuous Notch inhibition increases iPNEC specification iPNEC gene expression signature is highly concordant with primary human fetal PNECs Pulmonary neuroendocrine cells (PNECs) are sensory cells within the lung airway epithelia. Here, we provide a detailed protocol for generating induced PNECs (iPNECs) from human induced pluripotent stem cells (iPSCs). The cellular and molecular profile of iPNECs resembles primary human PNECs. Primary human PNECs are exceedingly rare, comprising only 1% of the adult lung. Therefore, a self-renewing source of patient-specific iPNECs facilitates the creation of reproducible human cellular models to study lung diseases characterized by PNEC dysfunction.
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影响因子：
23.9
作者：
Longmire, Tyler A.;Ikonomou, Laertis;Hawkins, Finn;Christodoulou, Constantina;Cao, Yuxia;Jean, J. C.;Kwok, Letty W.;Mou, Hongmei;Rajagopal, Jayaraj;Shen, Steven S.;Dowton, Anne A.;Serra, Maria;Weiss, Daniel J.;Green, Michael D.;Snoeck, Hans-Willem;Ramirez, Maria I.;Kotton, Darrell N.
通讯作者：
Kotton, Darrell N.
影响因子：
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作者：
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通讯作者：
Johnson, Jane E.
影响因子：
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作者：
Van Haute, Lindsey;De Block, Gert;De Rycke, Martine
通讯作者：
De Rycke, Martine
影响因子：
46.9
作者：
Gouon-Evans, Valerie;Boussemart, Lise;Keller, Gordon
通讯作者：
Keller, Gordon
DOI：
10.1165/rcmb.2005-0468oc
发表时间：
2006-09-01
影响因子：
6.4
作者：
Pan, Jie;Luk, Catherine;Yeger, Herman
通讯作者：
Yeger, Herman