A trio of viral proteins tunes aphid-plant interactions in Arabidopsis thaliana.
A trio of viral proteins tunes aphid-plant interactions in Arabidopsis thaliana.
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DOI：
10.1371/journal.pone.0083066
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Carr JP
中科院分区：
文献类型：
--
作者：
Westwood JH;Groen SC;Du Z;Murphy AM;Anggoro DT;Tungadi T;Luang-In V;Lewsey MG;Rossiter JT;Powell G;Smith AG;Carr JP
病毒诱导的对蚜虫取食的威慑被认为通过鼓励携带病毒的昆虫迁移远离受感染的植物来促进植物病毒传播。本文研究了蚜虫传播的黄瓜花叶病毒（CMV）感染对黄瓜花叶病毒（CMV）的天然寄主拟南芥（Arabidopsis thaliana）与桃蚜（Myzus persicae）（俗称“桃蚜”、“绿色桃蚜”）相互作用的影响。 用CMV株Fny（Fny-CMV）感染拟南芥（生态型Col-0）诱导蚜虫取食阻遏物4-甲氧基-吲哚-3-基-甲基硫代葡萄糖苷（4 MI 3 M）的生物合成。4 MI 3 M抑制蚜虫对韧皮部的摄取，从而阻止蚜虫定居。CMV 2b蛋白是抗病毒RNA沉默的抑制因子，其先前已涉及改变植物-蚜虫相互作用。它在感染宿主中的存在增强了CMV和其他四种病毒蛋白的积累。另一种病毒基因产物，2a蛋白（一种RNA依赖性RNA聚合酶），触发防御信号，导致4 MI 3 M积累增加。2b蛋白可以抑制ARGONAUTE 1（AGO 1），ARGONAUTE 1（AGO 1）是一种宿主因子，其既正向调节4 MI 3 M生物合成又负向调节对蚜虫有毒的物质的积累。然而，1a复制酶蛋白调节2b介导的AGO 1抑制，确保蚜虫被阻止进食，但不会中毒。CMV LS株系在拟南芥生态型Col-0中不诱导拒食。 2b蛋白对AGO 1的抑制可以作为诱杀装置，因为这将触发对蚜虫的抑制。然而，对于Fny-CMV，三种病毒蛋白（1a、2a和2b）的相互作用似乎平衡了病毒抑制抗病毒沉默的需要，同时诱导了被认为促进传播的温和抗性（抗异种作用）。CMV的菌株特异性的影响，对烟草蚜虫的相互作用，和Fny-CMV对这种植物的影响之间的差异和以前在烟草中看到的（抑制蚜虫的抗性）可能有重要的流行病学后果。
Virus-induced deterrence to aphid feeding is believed to promote plant virus transmission by encouraging migration of virus-bearing insects away from infected plants. We investigated the effects of infection by an aphid-transmitted virus, cucumber mosaic virus (CMV), on the interaction of Arabidopsis thaliana, one of the natural hosts for CMV, with Myzus persicae (common names: ‘peach-potato aphid’, ‘green peach aphid’). Infection of Arabidopsis (ecotype Col-0) with CMV strain Fny (Fny-CMV) induced biosynthesis of the aphid feeding-deterrent 4-methoxy-indol-3-yl-methylglucosinolate (4MI3M). 4MI3M inhibited phloem ingestion by aphids and consequently discouraged aphid settling. The CMV 2b protein is a suppressor of antiviral RNA silencing, which has previously been implicated in altering plant-aphid interactions. Its presence in infected hosts enhances the accumulation of CMV and the other four viral proteins. Another viral gene product, the 2a protein (an RNA-dependent RNA polymerase), triggers defensive signaling, leading to increased 4MI3M accumulation. The 2b protein can inhibit ARGONAUTE1 (AGO1), a host factor that both positively-regulates 4MI3M biosynthesis and negatively-regulates accumulation of substance(s) toxic to aphids. However, the 1a replicase protein moderated 2b-mediated inhibition of AGO1, ensuring that aphids were deterred from feeding but not poisoned. The LS strain of CMV did not induce feeding deterrence in Arabidopsis ecotype Col-0. Inhibition of AGO1 by the 2b protein could act as a booby trap since this will trigger antibiosis against aphids. However, for Fny-CMV the interplay of three viral proteins (1a, 2a and 2b) appears to balance the need of the virus to inhibit antiviral silencing, while inducing a mild resistance (antixenosis) that is thought to promote transmission. The strain-specific effects of CMV on Arabidopsis-aphid interactions, and differences between the effects of Fny-CMV on this plant and those seen previously in tobacco (inhibition of resistance to aphids) may have important epidemiological consequences.
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影响因子：
3.7
作者：
Harvey JJ;Lewsey MG;Patel K;Westwood J;Heimstädt S;Carr JP;Baulcombe DC
通讯作者：
Baulcombe DC
影响因子：
3.5
作者：
Gonzalez, Inmaculada;Martinez, Llucia;Canto, Tomas
通讯作者：
Canto, Tomas
DOI：
10.1007/1-4020-3780-5_14
发表时间：
2006-01-01
期刊：
NATURAL RESISTANCE MECHANISMS OF PLANTS TO VIRUSES
影响因子：
--
作者：
Handford, Michael G.;Carr, John P.
通讯作者：
Carr, John P.
影响因子：
2.9
作者：
De Vos, Martin;Van Oosten, Vivian R.;Pieterse, Corne M. J.
通讯作者：
Pieterse, Corne M. J.
影响因子：
64.8
作者：
Asai, T;Tena, G;Sheen, J
通讯作者：
Sheen, J