Bacterial pathogens deliver water- and solute-permeable channels to plant cells.
Bacterial pathogens deliver water- and solute-permeable channels to plant cells.
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DOI：
10.1038/s41586-023-06531-5
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发表时间：
2023-09
期刊：
影响因子：
64.8
通讯作者：
He, Sheng Yang
中科院分区：
文献类型：
--
作者：
Nomura, Kinya;Andreazza, Felipe;Cheng, Jie;Dong, Ke;Zhou, Pei;He, Sheng Yang
许多动物和植物病原性细菌使用III型分泌系统将效应蛋白递送到宿主细胞中。阐明这些效应蛋白在宿主细胞中的功能对于理解动物和植物中的感染性疾病至关重要。广泛保守的AvrE家族效应子，包括梨火疫病菌中的DspE和假单胞菌中的AvrE，在多种植物病原细菌的发病机制中具有核心作用。这些保守的效应子参与诱导“水浸”和宿主细胞死亡，这有助于感染组织中的细菌增殖。然而，三十年来，AvrE家族效应子的确切生物化学功能一直受到机制理解的阻碍。在这里，我们表明AvrE家族效应子折叠成类似于细菌孔蛋白的β桶结构。非洲爪蟾卵母细胞中AvrE和DspE的表达导致内向和外向电流、水渗透性和渗透压依赖性卵母细胞肿胀和破裂。脂质体重构证实，单独的DspE通道足以允许小分子如荧光素染料通过。基于DspE通道的预测孔径（15-20 μ m）的化学阻断剂的靶向筛选确定聚酰胺胺树状聚合物为DspE/AvrE通道的抑制剂。amylovora和P.因此，我们已经解开了一个重要的细菌效应子家族的生化功能，在细菌发病机制的研究中具有广泛的概念和实际意义。AvrE家族的细菌效应蛋白在植物细胞中充当膜通道，使得来自宿主细胞的水和溶质能够通过以支持病原体生长，并破坏宿主细胞的活力。
Many animal- and plant-pathogenic bacteria use a type III secretion system to deliver effector proteins into host cells. Elucidation of how these effector proteins function in host cells is critical for understanding infectious diseases in animals and plants. The widely conserved AvrE-family effectors, including DspE in Erwinia amylovora and AvrE in Pseudomonas syringae, have a central role in the pathogenesis of diverse phytopathogenic bacteria. These conserved effectors are involved in the induction of ‘water soaking’ and host cell death that are conducive to bacterial multiplication in infected tissues. However, the exact biochemical functions of AvrE-family effectors have been recalcitrant to mechanistic understanding for three decades. Here we show that AvrE-family effectors fold into a β-barrel structure that resembles bacterial porins. Expression of AvrE and DspE in Xenopus oocytes results in inward and outward currents, permeability to water and osmolarity-dependent oocyte swelling and bursting. Liposome reconstitution confirmed that the DspE channel alone is sufficient to allow the passage of small molecules such as fluorescein dye. Targeted screening of chemical blockers based on the predicted pore size (15–20 Å) of the DspE channel identified polyamidoamine dendrimers as inhibitors of the DspE/AvrE channels. Notably, polyamidoamines broadly inhibit AvrE and DspE virulence activities in Xenopus oocytes and during E. amylovora and P. syringae infections. Thus, we have unravelled the biochemical function of a centrally important family of bacterial effectors with broad conceptual and practical implications in the study of bacterial pathogenesis. The bacterial effector proteins of the AvrE family function as membrane channels in plant cells, enabling the passage of water and solutes from host cells to support pathogen growth, and disrupting the viability of the host cell.
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影响因子：
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