End-Functionalized Poly(vinylpyrrolidone) for Ligand Display in Lateral Flow Device Test Lines.
End-Functionalized Poly(vinylpyrrolidone) for Ligand Display in Lateral Flow Device Test Lines.
复制标题
DOI：
10.1021/acspolymersau.1c00032
复制
发表时间：
2022-04-13
期刊：
影响因子：
--
通讯作者：
Gibson MI
中科院分区：
文献类型：
--
作者：
Baker AN;Congdon TR;Richards SJ;Georgiou PG;Walker M;Dedola S;Field RA;Gibson MI
侧流设备是快速（通常成本低）的护理点诊断-经典的例子是家庭怀孕测试。测试线（固定相）通常通过抗体的物理吸附来制备，所述抗体结合分析物/抗原，例如病毒、毒素或激素。然而，对于检测单元是抗体没有内在要求，并且并入其他配体类别可以带来新的功能或检测能力。为了使其他（非蛋白质）配体被部署在侧流装置中，它们必须作为缀合物被物理吸附到固定相，其目前将是高分子量载体蛋白，其需要（具有挑战性的）化学选择性修饰和纯化。在这里，我们证明，聚（乙烯基吡咯烷酮），PVP，是一个候选人的聚合物，无蛋白质的测试线，由于其独特的平衡的水溶性（用于打印）和粘附到硝化纤维素固定相。采用RAFT聚合法制备了末端功能化的聚乙烯吡咯烷酮，并将生物素和半乳糖胺的模型捕获配体安装在聚乙烯吡咯烷酮上，随后固定在硝酸纤维素上。使用链霉亲和素和大豆凝集素，该聚合物测试线在流通和完全侧流形式中进行了验证，并且是用于安装捕获单元的“全聚合物”方法的第一个证明。这项工作说明了聚合物支架作为锚定剂的小分子捕获剂在下一代的强大的和模块化的侧流装置和大分子工程可能提供真实的好处的潜力。
Lateral flow devices are rapid (and often low cost) point-of-care diagnostics—the classic example being the home pregnancy test. A test line (the stationary phase) is typically prepared by the physisorption of an antibody, which binds to analytes/antigens such as viruses, toxins, or hormones. However, there is no intrinsic requirement for the detection unit to be an antibody, and incorporating other ligand classes may bring new functionalities or detection capabilities. To enable other (nonprotein) ligands to be deployed in lateral flow devices, they must be physiosorbed to the stationary phase as a conjugate, which currently would be a high-molecular-weight carrier protein, which requires (challenging) chemoselective modifications and purification. Here, we demonstrate that poly(vinylpyrrolidone), PVP, is a candidate for a polymeric, protein-free test line, owing to its unique balance of water solubility (for printing) and adhesion to the nitrocellulose stationary phase. End-functionalized PVPs were prepared by RAFT polymerization, and the model capture ligands of biotin and galactosamine were installed on PVP and subsequently immobilized on nitrocellulose. This polymeric test line was validated in both flow-through and full lateral flow formats using streptavidin and soybean agglutinin and is the first demonstration of an “all-polymer” approach for installation of capture units. This work illustrates the potential of polymeric scaffolds as anchoring agents for small-molecule capture agents in the next generation of robust and modular lateral flow devices and that macromolecular engineering may provide real benefit.
登录
查看更多内容
用于检测SARS-COV-2的基于聚糖的流通设备。
影响因子：
8.9
作者：
Baker AN;Richards SJ;Pandey S;Guy CS;Ahmad A;Hasan M;Biggs CI;Georgiou PG;Zwetsloot AJ;Straube A;Dedola S;Field RA;Anderson NR;Walker M;Grammatopoulos D;Gibson MI
通讯作者：
Gibson MI
DOI：
10.1007/978-1-59745-240-3_6
发表时间：
2009-01-01
期刊：
LATERAL FLOW IMMUNOASSAY
影响因子：
--
作者：
Mansfield, Michael A.
通讯作者：
Mansfield, Michael A.
DOI：
10.1186/s42506-021-00067-0
发表时间：
2021-02-17
影响因子：
--
作者：
Aung MN;Koyanagi Y;Yuasa M
通讯作者：
Yuasa M
影响因子：
4.6
作者：
Bell, Craig A.;Hedir, Guillaume G.;Dove, Andrew P.
通讯作者：
Dove, Andrew P.
影响因子：
7.3
作者：
Kaufman, Nichole E. M.;Meng, Qianli;Vicente, M. Graca H.
通讯作者：
Vicente, M. Graca H.