The physical state of lipid substrates provides transacylation specificity for tafazzin.
The physical state of lipid substrates provides transacylation specificity for tafazzin.
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DOI：
10.1038/nchembio.1064
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发表时间：
2012-10
影响因子：
14.8
通讯作者：
Epand, Richard M.
中科院分区：
文献类型：
--
作者：
Schlame, Michael;Acehan, Devrim;Berno, Bob;Xu, Yang;Valvo, Salvatore;Ren, Mindong;Stokes, David L.;Epand, Richard M.
心磷脂是一种线粒体磷脂，具有特征性酰基链组成，其取决于tafazzin（一种磷脂-溶血磷脂转酰酶）的功能，尽管该酶本身缺乏酰基特异性。我们孵育分离的tafazzin与各种混合物的磷脂和溶血磷脂，其特征在于脂质相的31 P-NMR，并测量新形成的分子种类质谱。仅在非双层脂质聚集体中观察到显著的转酰化，并且底物特异性对脂质相高度敏感。特别是，四氢萘油酰基-心磷脂，一种原型分子物种，仅在有利于倒六方相的条件下形成。在分离的线粒体中，<1%的脂质参与转酰化，表明tafazzin的作用仅限于特权脂质结构域。我们提出，tafazzin反应与非双层型脂质结构域发生在弯曲或半融合的膜区，和酰基特异性是由这些结构域的包装性能。
Cardiolipin is a mitochondrial phospholipid with a characteristic acyl chain composition that depends on the function of tafazzin, a phospholipid-lysophospholipid transacylase, although the enzyme itself lacks acyl specificity. We incubated isolated tafazzin with various mixtures of phospholipids and lysophospholipids, characterized the lipid phase by 31P-NMR, and measured newly formed molecular species by mass spectrometry. Significant transacylation was observed only in non-bilayer lipid aggregates and the substrate specificity was highly sensitive to the lipid phase. In particular, tetralinoleoyl-cardiolipin, a prototype molecular species, formed only under conditions that favor the inverted hexagonal phase. In isolated mitochondria, <1 percent of lipids participated in transacylations, suggesting that the action of tafazzin is limited to privileged lipid domains. We propose that tafazzin reacts with non-bilayer type lipid domains that occur in curved or hemifused membrane zones, and that acyl specificity is driven by the packing properties of these domains.
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影响因子：
4.8
作者：
Acehan, Devrim;Vaz, Frederic;Khuchua, Zaza
通讯作者：
Khuchua, Zaza
影响因子：
30.8
作者：
Bione, S;DAdamo, P;Toniolo, D
通讯作者：
Toniolo, D
DOI：
10.1016/0005-2736(82)90047-5
发表时间：
1982-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
DEKRUIJFF, B;NAYAR, R;CULLIS, PR
通讯作者：
CULLIS, PR
影响因子：
3.4
作者：
OTTEN, D;LOBBECKE, L;BEYER, K
通讯作者：
BEYER, K
影响因子：
3.4
作者：
Schlame, Michael;Blais, Steven;Neubert, Thomas A.
通讯作者：
Neubert, Thomas A.