Infrabarrels Are Layer 6 Circuit Modules in the Barrel Cortex that Link Long-Range Inputs and Outputs.
Infrabarrels Are Layer 6 Circuit Modules in the Barrel Cortex that Link Long-Range Inputs and Outputs.
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DOI：
10.1016/j.celrep.2017.11.049
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发表时间：
2017-12-12
期刊：
影响因子：
8.8
通讯作者：
Connors BW
中科院分区：
文献类型：
--
作者：
Crandall SR;Patrick SL;Cruikshank SJ;Connors BW
啮齿类动物的躯体感觉皮层包括定义明确的皮层柱（桶柱）的例子，这些皮层柱延伸到整个皮层深度，并由第4层（L4）中称为桶的离散神经元簇定义。使用细胞类型特异性Ntsr 1-Cre小鼠系，我们发现L 6包含“下桶”：与L4桶对齐的容易识别的单元。皮质丘脑（CT）神经元和它们的局部轴突聚集在下桶内，而皮质皮质（CC）神经元则密集在下桶之间。光遗传学实验表明，CC细胞从丘脑体感核团接收到强有力的输入，而CT细胞接收到的丘脑输入要弱得多。我们还发现CT神经元本质上不太兴奋，这表明突触和内在机制都导致了体内经常报道的CT神经元的低放电率。总之，下桶是离散的皮层电路模块，包含两个部分分离的兴奋性网络，将长距离丘脑输入与特定输出联系起来。第6层是新皮层的主要输入输出层，但缺乏其电路组织的关键原则。Crandall等人揭示了称为下桶的桶状结构，包括连接丘脑输入与特定输出的不同兴奋回路，从而为理解第6层的功能组织提供了框架。
The rodent somatosensory cortex includes well-defined examples of cortical columns—the barrel columns—that extend throughout the cortical depth and are defined by discrete clusters of neurons in layer 4 (L4) called barrels. Using the cell-type specific Ntsr1-Cre mouse line, we found that L6 contains “infrabarrels”: readily identifiable units that align with the L4 barrels. Corticothalamic (CT) neurons and their local axons cluster within the infrabarrels, whereas corticocortical (CC) neurons are densest between infrabarrels. Optogenetic experiments showed that CC cells received robust input from somatosensory thalamic nuclei, whereas CT cells received much weaker thalamic inputs. We also found that CT neurons are intrinsically less excitable, revealing that both synaptic and intrinsic mechanisms contribute to the low firing rates of CT neurons often reported in vivo. In summary, infrabarrels are discrete cortical circuit modules containing two partially separated excitatory networks that link long-distance thalamic inputs with specific outputs. Layer 6 is a major input-output layer of neocortex, but key principles of its circuit organization are lacking. Crandall et al. reveal barrel-like structures called infrabarrels that include distinct excitatory circuits linking thalamic inputs with specific outputs, thus providing a framework for understanding the functional organization of layer 6.
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影响因子：
25
作者：
Cruikshank, Scott J.;Lewis, Timothy J.;Connors, Barry W.
通讯作者：
Connors, Barry W.
影响因子：
5.3
作者：
Bokor, Hajnalka;Acsady, Laszlo;Deschenes, Martin
通讯作者：
Deschenes, Martin
影响因子：
9.8
作者：
Bureau, Ingrid;von Saint Paul, Francisca;Svoboda, Karel
通讯作者：
Svoboda, Karel
DOI：
10.1126/science.1236425
发表时间：
2013-06-28
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Constantinople CM;Bruno RM
通讯作者：
Bruno RM
影响因子：
5.3
作者：
Denman, Daniel J.;Contreras, Diego
通讯作者：
Contreras, Diego