Interdigitated paralemniscal and lemniscal pathways in the mouse barrel cortex.
Interdigitated paralemniscal and lemniscal pathways in the mouse barrel cortex.
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鼠标桶皮层中的同性恋和lem仪通路。
DOI：
10.1371/journal.pbio.0040382
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发表时间：
2006-11
期刊：
影响因子：
9.8
通讯作者：
Svoboda, Karel
中科院分区：
文献类型：
--
作者：
Bureau, Ingrid;von Saint Paul, Francisca;Svoboda, Karel
初级感觉皮质区通过多个丘脑通道接收信息。在啮齿类动物的须系统中，分别来自腹侧后内侧核（VPM）和后内侧核（POm）的lemnal和ponal丘脑皮质投影将不同类型的感觉信息传递给皮层。对于这些分离的活动流是如何在新皮层微电路中解析和整合的，我们知之甚少。本研究采用定量激光扫描光刺激技术，探讨了小鼠丘脑皮质的功能组织和桶状皮质内上升投影。为了绘制丘脑皮质的投影图，我们在刺激VPM或POm时记录了新皮质兴奋性神经元。(L)4、L5和L6A层神经元接受来自丘脑的密集输入（L4、L5B和L6A来自VPM， L5A来自POm），而L2/3层神经元很少接受丘脑输入。我们进一步绘制了从L4和L5A到L2/3的颅神经和平行神经回路。Lemniscal L4神经元在一个柱内靶向L3。肌旁L5A神经元靶向L1以下的浅表带（厚度60 μm）神经元，在小鼠桶状皮质中定义了一个功能不同的L2。L2神经元接受的输入来自于分布在多个柱上的颈神经L3细胞和颈旁L5A细胞。我们的数据表明，颈侧和颈旁信息被分离到指间皮层。利用激光扫描光刺激，作者绘制了小鼠体感觉皮层的丘脑皮层回路图，达到了前所未有的水平。它们揭示了不同皮层层之间丘脑通路的功能分离，这种分离也维持在皮层内回路中。
Primary sensory cortical areas receive information through multiple thalamic channels. In the rodent whisker system, lemniscal and paralemniscal thalamocortical projections, from the ventral posteromedial nucleus (VPM) and posterior medial nucleus (POm) respectively, carry distinct types of sensory information to cortex. Little is known about how these separate streams of activity are parsed and integrated within the neocortical microcircuit. We used quantitative laser scanning photostimulation to probe the organization of functional thalamocortical and ascending intracortical projections in the mouse barrel cortex. To map the thalamocortical projections, we recorded from neocortical excitatory neurons while stimulating VPM or POm. Neurons in layers (L)4, L5, and L6A received dense input from thalamus (L4, L5B, and L6A from VPM; and L5A from POm), whereas L2/3 neurons rarely received thalamic input. We further mapped the lemniscal and paralemniscal circuits from L4 and L5A to L2/3. Lemniscal L4 neurons targeted L3 within a column. Paralemniscal L5A neurons targeted a superficial band (thickness, 60 μm) of neurons immediately below L1, defining a functionally distinct L2 in the mouse barrel cortex. L2 neurons received input from lemniscal L3 cells and paralemniscal L5A cells spread over multiple columns. Our data indicate that lemniscal and paralemniscal information is segregated into interdigitated cortical layers. Using laser scanning photostimulation, the authors map thalamocortical circuits in the mouse somatosensory cortex at an unprecedented level. They reveal a functional segregation of thalamic pathways between distinct cortical layers that is maintained within the intracortical circuitry as well.
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影响因子：
25
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通讯作者：
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