Postnatal BDNF expression profiles in prefrontal cortex and hippocampus of a rat schizophrenia model induced by MK-801 administration.
Postnatal BDNF expression profiles in prefrontal cortex and hippocampus of a rat schizophrenia model induced by MK-801 administration.
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MK-801 诱导的大鼠精神分裂症模型前额皮质和海马的出生后 BDNF 表达谱
DOI：
10.1155/2010/783297
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发表时间：
2010
影响因子：
--
通讯作者：
Si T
中科院分区：
文献类型：
--
作者：
Guo C;Yang Y;Su Y;Si T
新生儿N-甲基-D-天冬氨酸（NMDA）受体阻断是精神分裂症的实验动物模型之一。本研究旨在探讨脑源性神经营养因子（brain-derived neurotrophic factor，BDNF）在大鼠青春期和成年期不同脑区的长期表达谱及其与“精神分裂样”行为的相关性。在出生后第5 - 14天（PND）对雌性Sprague-Dawley大鼠给予NMDA受体拮抗剂MK 801。在PND 42和PND 77进行旷场测试以检查当前模型的有效性。在PND 15、PND 42和PND 77分析海马和前额叶皮质（PFC）中的BDNF蛋白水平。结果表明，MK-801持续刺激新生鼠的自发活动和BDNF表达，BDNF表达的变化在不同发育阶段和脑区之间存在差异。然而，这些研究结果提供了神经化学证据表明，在大脑发育过程中的NMDA受体的阻断导致BDNF表达的长期变化，并可能有助于本精神分裂症动物模型的神经行为病理学。对BDNF的作用机制的进一步研究将有助于更好地理解精神分裂症的病理生理机制。
Neonatal blockade of N-methyl-D-aspartic acid (NMDA) receptors represents one of experimental animal models for schizophrenia. This study is to investigate the long-term brain-derived neurotrophic factor (BDNF) expression profiles in different regions and correlation with “schizophrenia-like” behaviors in the adolescence and adult of this rat model. The NMDA receptor antagonist MK801 was administered to female Sprague-Dawley rats on postnatal days (PND) 5 through 14. Open-field test was performed on PND 42, and PND 77 to examine the validity of the current model. BDNF protein levels in hippocampus and prefrontal cortex (PFC) were analyzed on PND 15, PND 42, and PND 77. Results showed that neonatal challenge with MK-801 persistently elevated locomotor activity as well as BDNF expression; the alterations in BDNF expression varied at different developing stages and among brain regions. However, these findings provide neurochemical evidence that the blockade of NMDA receptors during brain development results in long-lasting alterations in BDNF expression and might contribute to neurobehavioral pathology of the present animal model for schizophrenia. Further study in the mechanisms and roles of the BDNF may lead to better understanding of the pathophysiology of schizophrenia.
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DOI：
10.1016/s0140-6736(15)01121-6
发表时间：
2016-07-02
期刊：
Lancet (London, England)
影响因子：
--
作者：
Owen MJ;Sawa A;Mortensen PB
通讯作者：
Mortensen PB
影响因子：
5.3
作者：
Adams, B;Moghaddam, B
通讯作者：
Moghaddam, B
影响因子：
5
作者：
Su, Yun-Ai;Si, Tian-Mei;Liang, Jian-Hui
通讯作者：
Liang, Jian-Hui
影响因子：
11
作者：
Takahashi, M;Shirakawa, O;Nawa, H
通讯作者：
Nawa, H
影响因子：
3.3
作者：
Wang, C;McInnis, J;Johnson, KM
通讯作者：
Johnson, KM