Molecular epidemiology and antibiotic susceptibility of livestock Brucella melitensis isolates from Naryn Oblast, Kyrgyzstan.
Molecular epidemiology and antibiotic susceptibility of livestock Brucella melitensis isolates from Naryn Oblast, Kyrgyzstan.
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来自吉尔吉斯斯坦Naryn Oblast中的牲畜布鲁氏菌融合物分离株的分子流行病学和抗生素敏感性。
DOI：
10.1371/journal.pntd.0002047
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发表时间：
2013
影响因子：
3.8
通讯作者：
Zinsstag J
中科院分区：
文献类型：
--
作者：
Kasymbekov J;Imanseitov J;Ballif M;Schürch N;Paniga S;Pilo P;Tonolla M;Benagli C;Akylbekova K;Jumakanova Z;Schelling E;Zinsstag J
吉尔吉斯斯坦人间布鲁氏菌病的发病率在过去几年中一直在上升，并被确定为需要在牲畜群体中采取最紧急控制措施的优先疾病。吉尔吉斯斯坦布鲁氏菌分离株的最新种属鉴定是在20世纪60年代进行的，并调查了牛种布鲁氏菌B的传播情况。melitensis，B. ovis和B.猪。然而，缺乏这方面经验的佐证数据和文件。因此，布鲁氏菌的分型。在吉尔吉斯斯坦，为了了解主要传播途径和采取有效的布鲁氏菌病控制政策，有必要确定最重要的宿主物种。总的来说，17个B。研究了从纳伦省分离的羊和牛流产胎儿中分离的羊种布氏菌菌株。所有菌株均对甲氧苄啶-磺胺甲恶唑、庆大霉素、利福平、氧氟沙星、链霉素、多西环素和环丙沙星敏感。多位点可变数目串联重复序列分析显示遗传多样性较低。吉尔吉斯菌株似乎与布鲁氏杆菌全球性遗传的东地中海群有遗传关联。我们发现并确认了B的传播。Melitensis与牛的亲缘关系较近，B.从共享同一牧场的绵羊中分离的羊种牛痘病毒株。布鲁氏菌病是一种细菌性疾病，可导致牛、绵羊和山羊流产。它可通过直接传播和食用未经处理的牛奶传播给人类。在过去的几十年里，布鲁氏菌病在吉尔吉斯斯坦变得越来越频繁，控制布鲁氏菌病已成为优先事项。了解细菌菌株循环是重要的了解传播和干预措施的选择。吉尔吉斯斯坦布鲁氏菌的最新鉴定可追溯到20世纪60年代。本文报道了从纳伦州分离的17株羊种布鲁氏菌的分子特征。菌株主要分离自绵羊，但也分离自牛。所有菌株均对一系列抗生素敏感。因此，我们确定并确认了B的传播。羊中的羊种可能是最重要的宿主物种。结果表明，B与B之间有较近的亲缘关系。从与绵羊共享同一牧场的牛中分离的羊种布氏菌菌株。我们的研究结果支持在吉尔吉斯斯坦进行大规模牲畜疫苗接种的战略。需要进一步的研究来确定人类中最重要的循环菌株。
The incidence of human brucellosis in Kyrgyzstan has been increasing in the last years and was identified as a priority disease needing most urgent control measures in the livestock population. The latest species identification of Brucella isolates in Kyrgyzstan was carried out in the 1960s and investigated the circulation of Brucella abortus, B. melitensis, B. ovis, and B. suis. However, supporting data and documentation of that experience are lacking. Therefore, typing of Brucella spp. and identification of the most important host species are necessary for the understanding of the main transmission routes and to adopt an effective brucellosis control policy in Kyrgyzstan. Overall, 17 B. melitensis strains from aborted fetuses of sheep and cattle isolated in the province of Naryn were studied. All strains were susceptible to trimethoprim-sulfamethoxazole, gentamicin, rifampin, ofloxacin, streptomycin, doxycycline, and ciprofloxacin. Multilocus variable number tandem repeat analysis showed low genetic diversity. Kyrgyz strains seem to be genetically associated with the Eastern Mediterranean group of the Brucella global phylogeny. We identified and confirmed transmission of B. melitensis to cattle and a close genetic relationship between B. melitensis strains isolated from sheep sharing the same pasture. Brucellosis is a bacterial disease causing abortion in cattle, sheep, and goats. It is transmissible to humans by direct transmission and the consumption of untreated milk. Brucellosis has become more and more frequent in Kyrgyzstan in the last decades, and its control has been made a priority. Knowing the bacterial strain circulating is important for the understanding of the transmission and the selection of interventions. The latest identification of Brucella in Kyrgyzstan dates from the 1960s. We report the molecular characterization 17 strains identified as Brucella melitensis from Naryn oblast. Strains were mainly isolated from sheep but also from cattle. All strains were susceptible to a series of antibiotics. We hence identified and confirmed transmission of B. melitensis among sheep which is likely the most important host species. We found close genetic relationship between B. melitensis strains isolated from cattle sharing the same pasture with sheep. Our results support the strategy of pursuing a mass vaccination of livestock in Kyrgyzstan. Further research is needed to identify the most important circulating strains in humans.
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影响因子：
4.2
作者：
Le Flèche P;Jacques I;Grayon M;Al Dahouk S;Bouchon P;Denoeud F;Nöckler K;Neubauer H;Guilloteau LA;Vergnaud G
通讯作者：
Vergnaud G
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作者：
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通讯作者：
Schelling, Esther
影响因子：
4.2
作者：
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通讯作者：
Vergnaud, Gilles