Plastid ribosomal protein LPE2 is involved in photosynthesis and the response to C/N balance in Arabidopsis thaliana.
Plastid ribosomal protein LPE2 is involved in photosynthesis and the response to C/N balance in Arabidopsis thaliana.
复制标题
拟南芥质体核糖体蛋白 LPE2 参与光合作用和 C/N 平衡响应
DOI：
10.1111/jipb.12907
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发表时间：
2020-09
影响因子：
11.4
通讯作者：
Jin HL
中科院分区：
文献类型：
--
作者：
Dong X;Duan S;Wang HB;Jin HL
摘要植物细胞碳氮平衡必须紧密协调，才能维持最佳生长发育。在叶绿体中，光合作用将无机碳转化为有机碳，这对维持植物细胞中的碳含量很重要。然而，对叶绿体在C/N平衡中的作用知之甚少。在这里，我们确定了一个核编码的蛋白质低光合效率2（LPE 2），它是拟南芥光合作用和C/N平衡所必需的。LPE 2特异性地定位于叶绿体中。两种功能丧失突变体lpe 2 - 1和lpe 2 - 2表现出较低的光合活性，其特征在于电子传递较慢，PSII量子产率低于野生型。值得注意的是，预测LPE 2编码质体核糖体蛋白S21（RPS 21）。LPE 2的缺失显著影响了类囊体膜的组成和质体蛋白的积累，但对质体基因的转录没有明显影响。更有趣的是，转录组分析表明，LPE 2的缺失改变了细胞核中C和N反应相关基因的表达，这一点已被定量真实的时间-聚合酶链反应证实。LPE 2的缺乏在生理水平上抑制了C/N平衡的响应。综上所述，我们的研究结果表明，LPE 2在光合作用和响应C/N平衡中起着双重作用。
Abstract The balance between cellular carbon (C) and nitrogen (N) must be tightly coordinated to sustain optimal growth and development in plants. In chloroplasts, photosynthesis converts inorganic C to organic C, which is important for maintenance of C content in plant cells. However, little is known about the role of chloroplasts in C/N balance. Here, we identified a nuclear‐encoded protein LOW PHOTOSYNTHETIC EFFICIENCY2 (LPE2) that it is required for photosynthesis and C/N balance in Arabidopsis. LPE2 is specifically localized in the chloroplast. Both loss‐of‐function mutants, lpe2‐1 and lpe2‐2, showed lower photosynthetic activity, characterized by slower electron transport and lower PSII quantum yield than the wild type. Notably, LPE2 is predicted to encode the plastid ribosomal protein S21 (RPS21). Deficiency of LPE2 significantly perturbed the thylakoid membrane composition and plastid protein accumulation, although the transcription of plastid genes is not affected obviously. More interestingly, transcriptome analysis indicated that the loss of LPE2 altered the expression of C and N response related genes in nucleus, which is confirmed by quantitative real‐time‐polymerase chain reaction. Moreover, deficiency of LPE2 suppressed the response of C/N balance in physiological level. Taken together, our findings suggest that LPE2 plays dual roles in photosynthesis and the response to C/N balance.
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