Colocalization of β‐adrenergic receptors and caveolin within the plasma membrane
Colocalization of β‐adrenergic receptors and caveolin within the plasma membrane
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质膜内 β-肾上腺素能受体和小窝蛋白的共定位
DOI：
--
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Y. Ishikawa
中科院分区：
文献类型：
--
作者：
C. Schwencke;S. Okumura;Manabu Yamamoto;Y. Geng;Y. Ishikawa
β-肾上腺素能受体信号的快速扩增涉及从受体到腺苷环化酶等多个信号分子的顺序激活。激动剂诱导的信号分子之间相互作用的主流观点是基于蛋白质之间的随机碰撞，这些蛋白质在质膜中自由扩散。最近在小窝中发现了G蛋白α-和βγ-亚基及其与小窝的功能相互作用，提示小窝可能参与G蛋白偶联信号转导。我们研究了静息条件下β-肾上腺素能受体与小窝蛋白的潜在相互作用。β-1和β-2-肾上腺素能受体在COS7细胞中有重组过表达。用免洗涤剂蔗糖梯度离心法分离小窝。β1-和β2-肾上腺素能受体定位于与小窝蛋白相同的梯度组分，其中还检测到Gs、α和βγ-亚基。免疫荧光显微镜显示β-肾上腺素能受体与小凹蛋白共存，表明β-肾上腺素能受体在细胞膜上呈非随机分布。利用多组氨酸标记的重组蛋白，β肾上腺素能受体与小窝蛋白结合，表明它们是物理结合的。我们的结果表明，除了笼状蛋白包被的小窝，小窝可能是另一个划分β-肾上腺素能受体的质膜微区。J.细胞。生物化学。75岁，-72,1999年；©1999 Wiley-Liss，Inc.
The rapid amplification of β‐adrenergic receptor signaling involves the sequential activation of multiple signaling molecules ranging from the receptor to adenylyl cyclase. The prevailing view of the agonist‐induced interaction between signaling molecules is based on random collisions between proteins that diffuse freely in the plasma membrane. The recent identification of G protein α‐ and βγ‐subunits in caveolae and their functional interaction with caveolin suggests that caveolae may participate in G protein‐coupled signaling. We have investigated the potential interaction of β‐adrenergic receptors with caveolin under resting conditions. β1‐ and β2‐adrenergic receptors were recombinantly overexpressed in COS‐7 cells. Caveolae were isolated using the detergent‐free sucrose gradient centrifugation method. β1‐ and β2‐adrenergic receptors were localized in the same gradient fractions as caveolin, where Gsα‐ and βγ‐subunits were detected as well. Immunofluorescence microscopy demonstrated the colocalization of β‐adrenergic receptors with caveolin, indicating a nonrandom distribution of β‐adrenergic receptors in the plasma membrane. Using polyhistidine‐tagged recombinant proteins, β‐adrenergic receptors were copurified with caveolin, suggesting that they were physically bound. Our results suggest that, in addition to clathrin‐coated pits, caveolae may act as another plasma membrane microdomain to compartmentalize β‐adrenergic receptors. J. Cell. Biochem. 75:64–72, 1999. © 1999 Wiley‐Liss, Inc.
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DOI：
10.1073/pnas.91.24.11728
发表时间：
1994-11-22
影响因子：
11.1
作者：
CHUN, MY;LIYANAGE, UK;LODISH, HF
通讯作者：
LODISH, HF
通过放射性配体结合位点的定量超微结构放射自显影描绘出 β2-肾上腺素能受体的亚细胞分布。
影响因子：
20.1
作者：
Saffitz,JE;Liggett,SB
通讯作者：
Liggett,SB
分离成年大鼠心室肌细胞中 β-肾上腺素能受体-腺苷酸环化酶途径信号成分的定量和放大。
DOI：
10.1042/bj3110075
发表时间：
1995
期刊：
The Biochemical journal
影响因子：
--
作者：
Post,SR;Hilal-Dandan,R;Urasawa,K;Brunton,LL;Insel,PA
通讯作者：
Insel,PA
影响因子：
3.3
作者：
Huang, CF;Hepler, JR;Mumby, SM
通讯作者：
Mumby, SM
一种新型的腺苷酸环化酶 (AC) 肽抑制剂。
DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kawabe,J;Ebina,T;Ismail,S;Kitchen,DB;Homcy,CJ;Ishikawa,Y
通讯作者：
Ishikawa,Y