Biological characteristics of a new human glioma cell line transformed into A2B5(+) stem cells.
Biological characteristics of a new human glioma cell line transformed into A2B5(+) stem cells.
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DOI：
10.1186/s12943-015-0343-z
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发表时间：
2015-04-02
期刊：
影响因子：
37.3
通讯作者：
Zhou Y
中科院分区：
文献类型：
--
作者：
Li Y;Wang H;Sun T;Chen J;Guo L;Shen H;Du Z;Zhou Y
建立胶质瘤新细胞系SHG-139，对其表型、致瘤性、病理特征、衍生干细胞SHG139S进行研究。免疫组化法检测患者和小鼠肿瘤组织中SHG-139和SHG-139S的表达情况。进行SHG-139原代培养，观察细胞增殖、细胞周期及遗传特性。进行MiRNA（微RNA）和LncRNA（长链非编码RNA）微阵列检测。我们发现胶质瘤组织中A2B5（胶质前体神经节苷）、GFAP（胶质纤维酸性蛋白）、S-100（酸性钙结合蛋白）、VEGF（血管内皮生长因子）、VEGFR（血管内皮生长因子受体）呈阳性，Ki-67（核相关抗原）呈阴性。SHG-139在24h内增殖显著；染色体总数为68条；G1期SHG-139和SHG-139S细胞比例最高。SHG-139细胞A2B5、半乳糖脑苷（GalC）、GFAP、S-100、Vimentin阳性，SHG-139S细胞A2B5、Nestin、NG2（神经胶质抗原2）阳性，Vimentin、IDHR132H（异柠檬酸脱氢酶）阴性；细胞很少染色CD133 （Cluster of differentiation133）。SHG-139颅内异种移植物表达GFAP，但未观察到明显的少突胶质细胞瘤。在SHG-139S异种移植物中，表达GFAP和S-100，未检测到CD133；肿瘤边缘可见少量A2B5+细胞，为典型的少突胶质细胞瘤。此外，SHG-139S异种移植物肿瘤比SHG-139更具侵袭性。抗小鼠CD31 （Cluster of differentiation31）染色显示异种移植瘤与正常脑组织交界处有小鼠血管；抗人CD34 （Cluster of differentiation34）染色为阴性。SHG139S的生物芯片技术显示，多个miRNA和lncRNA在SHG139和SHG139S中表达不同。SHG-139是一株星形胶质瘤细胞系，产生干细胞SHG-139S。SHG-139S细胞构成A2B5+/CD133−GSC亚群。本文的在线版本（doi:10.1186/s12943-015-0343-z）包含补充材料，仅供授权用户使用。
The new glioma cell line SHG-139 was established and its phenotype, tumorigenicity, pathological characteristics, derived stem cells SHG139S were studied. Immunohistochemistry was used to assess expressions in the patient and mouse tumor tissues, SHG-139 and SHG-139S. Primary SHG-139 culture was performed, cell proliferation, cell cycle and genetic characteristics were assessed. MiRNA (Micro RNA) and LncRNA (Long non-coding RNA) microarray was performed. We found that the glioma tissue was positive for A2B5 (Glial precursors ganglioside), GFAP (Glial fibrillary acidic protein), S-100 (Acid calcium bingding protein), VEGF (Vascular endothelial growth factor), VEGFR (Vascular endothelial growth factor receptor) and negative for Ki-67 (Nuclcar- associated antigen). SHG-139 proliferated significantly within 24h; its total number of chromosomes was 68; ratios of SHG-139 and SHG-139S cells in G1 phase were highest. SHG-139 cells were positive for A2B5, GalC (Galactocerebrosides), GFAP, S-100 and Vimentin, while SHG-139S cells were positive for A2B5, Nestin, and NG2 (Neuron-glia antigen2), and negative for Vimentin and IDHR132H (Isocitrate dehydrogenase); cells rarely stained for CD133 (Cluster of differentiation133). SHG-139 intracranial xenografts expressed GFAP, but no overt oligodendroglioma was observed. In SHG-139S xenografts, GFAP and S-100 were expressed, while CD133 was not detected; a few A2B5+ cells were found at tumor edges, and typical oligodendroglioma were obtained. In addition, SHG-139S xenograft tumors were more aggressive than those of SHG-139. Anti-mouse CD31 (Cluster of differentiation31) staining revealed murine vessels at the border between xenograft tumor and normal brain tissue; Anti-human CD34 (Cluster of differentiation34) staining was negative. Biochip technology of SHG139S showed several miRNA and lncRNA were differently expressed in SHG139 and SHG139S. SHG-139 was an astroglioma cell line which yielded stem cells SHG-139S. SHG-139S cells constituted an A2B5+/CD133− GSC subgroup. The online version of this article (doi:10.1186/s12943-015-0343-z) contains supplementary material, which is available to authorized users.
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DOI：
10.1016/0360-3016(81)90136-x
发表时间：
1981-01-01
影响因子：
7
作者：
HENDERSON, SD;KIMLER, BF;MORANTZ, RA
通讯作者：
MORANTZ, RA
影响因子：
4.8
作者：
Ogden, Alfred T.;Waziri, Allen E.;Bruce, Jeffrey N.
通讯作者：
Bruce, Jeffrey N.
影响因子：
5.2
作者：
Ghods, Ali Jourabcih;Irvin, Dwain;Yu, John S.
通讯作者：
Yu, John S.
影响因子：
3
作者：
Al-Mayhani, Talal M. Fael;Ball, Siolian L. R.;Watts, Colin
通讯作者：
Watts, Colin
影响因子：
3.7
作者：
Thirant C;Bessette B;Varlet P;Puget S;Cadusseau J;Tavares Sdos R;Studler JM;Silvestre DC;Susini A;Villa C;Miquel C;Bogeas A;Surena AL;Dias-Morais A;Léonard N;Pflumio F;Bièche I;Boussin FD;Sainte-Rose C;Grill J;Daumas-Duport C;Chneiweiss H;Junier MP
通讯作者：
Junier MP