Circ-BPTF promotes bladder cancer progression and recurrence through the miR-31-5p/RAB27A axis.
Circ-BPTF promotes bladder cancer progression and recurrence through the miR-31-5p/RAB27A axis.
复制标题
Circ-BPTF通过miR-31-5p/RAB27A轴促进膀胱癌进展和复发
DOI：
10.18632/aging.101520
复制
发表时间：
2018-08-09
期刊：
影响因子：
--
通讯作者：
Lin T
中科院分区：
文献类型：
--
作者：
Bi J;Liu H;Cai Z;Dong W;Jiang N;Yang M;Huang J;Lin T
Circ-BPTF（hsa_circ_0000799）是一种来源于BPTF外显子的新型环状RNA。虽然BPTF是一个被广泛研究的前体基因，但circ-BPTF的特征和功能尚未报道。在这里，我们表明，与非癌组织和细胞系相比，膀胱癌（BCa）组织和细胞系中circ-BPTF的表达增加。结论：circ-BPTF表达水平高的BCa患者肿瘤分级高，预后差。在功能上，敲低circ-BPTF在体外和体内抑制肿瘤进展。在机制上，证实了circ-BPTF的靶microRNA是miR-31- 5 p，并且miR-31- 5 p模拟物部分逆转了circ-BPTF的作用。此外，预测并显示RAB 27 A是miR-31- 5 p的靶点，并且circ-BPTF减弱了miR-31- 5 p的抗癌作用，从而增强了RAB 27 A的表达。总之，我们的发现揭示了circ-BPTF通过miR-31- 5 p/RAB 27 A轴促进BCa进展，表明circ-BPTF可能是BCa治疗的潜在靶标。
Circ-BPTF (hsa_circ_0000799) is a novel circular RNA derived from BPTF exons. Although BPTF is a well-studied predecessor gene, the characteristics and functions of circ-BPTF have not yet been reported. Here, we show that expression of circ-BPTF is increased in bladder cancer (BCa) tissues and cell lines compared with noncancerous tissues and cell lines. Consistently, BCa patients with higher expression levels of circ-BPTF were found to have higher tumor grades and poorer prognosis. Functionally, knockdown of circ-BPTF inhibited tumor progression in vitro and in vivo. Mechanistically, a target microRNA of circ-BPTF was confirmed to be miR-31-5p, and miR-31-5p mimics partially reversed the effect of circ-BPTF. Furthermore, RAB27A was predicted and shown to be a target of miR-31-5p, and circ-BPTF attenuated the anti-oncogenic effect of miR-31-5p and consequently enhanced RAB27A expression. In summary, our findings reveal that circ-BPTF promotes BCa progression through the miR-31-5p/RAB27A axis, suggesting that circ-BPTF may be a potential target for BCa treatment.
登录
查看更多内容
影响因子：
--
作者：
Dai M;Lu JJ;Guo W;Yu W;Wang Q;Tang R;Tang Z;Xiao Y;Li Z;Sun W;Sun X;Qin Y;Huang W;Deng WG;Wu T
通讯作者：
Wu T
影响因子：
--
作者：
Liu J;Gong X;Zhu X;Xue D;Liu Y;Wang P
通讯作者：
Wang P
circRNA生物合成的调控
影响因子：
4.1
作者：
Chen, Ling-Ling;Yang, Li
通讯作者：
Yang, Li
环状RNA MYLK作为竞争性内源RNA通过调节VEGFA/VEGFR2信号通路促进膀胱癌进展
影响因子：
9.7
作者：
Zhong, Zhenyu;Huang, Mengge;Chen, Junxia
通讯作者：
Chen, Junxia
影响因子：
5.3
作者：
Okholm TLH;Nielsen MM;Hamilton MP;Christensen LL;Vang S;Hedegaard J;Hansen TB;Kjems J;Dyrskjøt L;Pedersen JS
通讯作者：
Pedersen JS