The enhancement effect of estradiol on contextual fear conditioning in female mice.
The enhancement effect of estradiol on contextual fear conditioning in female mice.
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DOI：
10.1371/journal.pone.0197441
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Tomihara K
中科院分区：
文献类型：
--
作者：
Matsumoto YK;Kasai M;Tomihara K
几项研究报道了雌激素对雌性啮齿动物恐惧条件反射的调节作用。然而，这些研究使用了不同的剂量、持续时间和/或给药方法，并报告了不一致的结果。为了阐明雌激素对恐惧条件反射的影响，我们研究了不同剂量和持续时间的雌二醇给药对卵巢切除（OVX）小鼠在情境恐惧条件反射过程中冻结行为的影响。在实验1中，OVX ICR小鼠接受单次皮下（s.c.）注射油媒介物（对照，0.1ml芝麻油）或不同剂量（0.5 μg/0.1ml、5 μg/0.1ml或50 μg/0.1ml）的17β-雌二醇-3-苯甲酸酯（EB）。在EB处理后两天进行恐惧条件反射，并在第二天测试小鼠的学习恐惧反应。在实验2中，OVX雌性小鼠接受s.c.植入硅橡胶胶囊（I.D. 1.98× 20.0 mm），含有溶剂或不同剂量（0.05 μg/0.1 ml、0.5 μg/0.1 ml、5 μg/0.1 ml、50 μg/0.1 ml）的EB。植入后两周，进行恐惧条件反射。在调节后24小时进行的测试期间，与对照组或低剂量组相比，高剂量EB组在两个实验中均显示出较长的冷冻时间和较低的运动活性。在实验3中，测量了像实验2中那样处理的小鼠的血清雌二醇浓度;血清雌二醇水平根据给予的EB剂量线性增加。结果表明，无论治疗持续时间如何，用高剂量EB治疗的小鼠表现出增强的恐惧学习。由于女性在围怀孕期对情绪障碍的脆弱性增加，在此期间雌激素水平很高，因此高剂量EB组的结果对于理解这些疾病中涉及的激素机制可能很重要。
Several studies have reported regulatory effects of estrogens on fear conditioning in female rodents. However, these studies used different doses, durations, and/or administration methods, and reported inconsistent results. To clarify the effect of estrogen on fear conditioning, we investigated the effects of different doses and durations of estradiol administration on freezing behavior during contextual fear conditioning in ovariectomized (OVX) mice. In Experiment 1, OVX ICR mice received a single subcutaneous (s.c.) injection of either oil vehicle (control, 0.1 ml sesame oil) or varied doses (0.5 μg/0.1 ml, 5 μg/0.1 ml, or 50 μg/0.1 ml) of 17β-estradiol-3-benzoate (EB). Fear conditioning was conducted two days post-EB treatment, and the mice were tested for the learned fear response the following day. In Experiment 2, OVX female mice received an s.c. implantation of a Silastic capsule (I.D. 1.98 × 20.0 mm) containing either vehicle or varied doses (0.05 μg/0.1 ml, 0.5 μg/0.1 ml, 5 μg/0.1 ml, 50 μg/0.1 ml) of EB. Two weeks after implantation, fear conditioning was conducted. During the tests conducted 24 h after conditioning, the high dose EB group showed longer freezing times in both experiments, and lower locomotor activity compared to the control or lower dose groups. In Experiment 3, serum estradiol concentrations of the mice that were treated like those in Experiment 2, were measured; the serum levels of estradiol increased linearly according to the dose of EB administered. The results suggest that mice treated with a high dose of EB exhibit enhanced fear learning, regardless of treatment duration. As a woman’s vulnerability to emotional disorders increases in the peripregnancy period, during which estrogen levels are high, the results from the high-dose EB groups may be important for understanding the hormonal mechanisms involved in these disorders.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
Lymer, Jennifer;Robinson, Alana;Choleris, Elena
通讯作者：
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影响因子：
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作者：
Gasbarri, Antonella;Pompili, Assunta;Tomaz, Carlos
通讯作者：
Tomaz, Carlos
影响因子：
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作者：
Mödder, UIL;Riggs, BL;Khosla, S
通讯作者：
Khosla, S
DOI：
10.1348/14760830260569265
发表时间：
2003-03-01
影响因子：
3.4
作者：
Crawley, RA;Dennison, K;Carter, C
通讯作者：
Carter, C