Human kidney is a target for novel severe acute respiratory syndrome coronavirus 2 infection.
Human kidney is a target for novel severe acute respiratory syndrome coronavirus 2 infection.
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人类肾脏是新型严重急性呼吸综合征冠状病毒2感染的目标
DOI：
10.1038/s41467-021-22781-1
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发表时间：
2021-05-04
影响因子：
16.6
通讯作者：
Chen Y
中科院分区：
文献类型：
--
作者：
Diao B;Wang C;Wang R;Feng Z;Zhang J;Yang H;Tan Y;Wang H;Wang C;Liu L;Liu Y;Liu Y;Wang G;Yuan Z;Hou X;Ren L;Wu Y;Chen Y
严重急性呼吸综合征冠状病毒2（SARS - CoV - 2）是否能直接感染人类肾脏从而导致急性肾损伤（AKI）尚不清楚。在此，我们对85例实验室确诊的2019冠状病毒病（COVID - 19）患者的临床参数进行了回顾性分析；此外，还对另外6例进行了尸检的COVID - 19患者的肾脏组织病理学进行了研究。我们发现27%（23/85）的患者出现了AKI。老年患者以及伴有合并症（高血压和心力衰竭）的病例更容易发生AKI。苏木精 - 伊红染色显示，COVID - 19尸检的肾脏有中度至重度的肾小管损伤。原位杂交检测表明病毒RNA在肾小管中积聚。免疫组织化学显示核衣壳蛋白和刺突蛋白沉积在肾小管中，免疫荧光双染色显示这两种抗原都局限于血管紧张素转换酶 - II阳性的肾小管。SARS - CoV - 2感染引发了缺氧损伤相关分子的表达，包括感染肾小管中的DP2和前列腺素D合酶。此外，它还增强了CD68 +巨噬细胞向肾小管间质的浸润，并且还观察到补体C5b - 9在肾小管上的沉积。这些结果表明，SARS - CoV - 2直接感染人类肾脏，介导肾小管发病机制和AKI。
严重急性呼吸综合征冠状病毒2（SARS - CoV - 2）可导致急性肾损伤。作者描述SARS - CoV - 2可直接感染人类肾脏，可能介导肾小管发病机制。
It is unclear whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can directly infect human kidney, thus leading to acute kidney injury (AKI). Here, we perform a retrospective analysis of clinical parameters from 85 patients with laboratory-confirmed coronavirus disease 2019 (COVID-19); moreover, kidney histopathology from six additional COVID-19 patients with post-mortem examinations was performed. We find that 27% (23/85) of patients exhibited AKI. The elderly patients and cases with comorbidities (hypertension and heart failure) are more prone to develop AKI. Haematoxylin & eosin staining shows that the kidneys from COVID-19 autopsies have moderate to severe tubular damage. In situ hybridization assays illustrate that viral RNA accumulates in tubules. Immunohistochemistry shows nucleocapsid and spike protein deposits in the tubules, and immunofluorescence double staining shows that both antigens are restricted to the angiotensin converting enzyme-II-positive tubules. SARS-CoV-2 infection triggers the expression of hypoxic damage-associated molecules, including DP2 and prostaglandin D synthase in infected tubules. Moreover, it enhances CD68+ macrophages infiltration into the tubulointerstitium, and complement C5b-9 deposition on tubules is also observed. These results suggest that SARS-CoV-2 directly infects human kidney to mediate tubular pathogenesis and AKI.
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影响因子：
19.6
作者：
Chu KH;Tsang WK;Tang CS;Lam MF;Lai FM;To KF;Fung KS;Tang HL;Yan WW;Chan HW;Lai TS;Tong KL;Lai KN
通讯作者：
Lai KN
影响因子：
13.2
作者：
Chan, Jasper Fuk-Woo;Kok, Kin-Hang;Yuen, Kwok-Yung
通讯作者：
Yuen, Kwok-Yung
影响因子：
168.9
作者：
Huang, Chaolin;Wang, Yeming;Cao, Bin
通讯作者：
Cao, Bin
影响因子：
168.9
作者：
Chan, Jasper Fuk-Woo;Yuan, Shuofeng;Yuen, Kwok-Yung
通讯作者：
Yuen, Kwok-Yung
多器官感染和SAR的发病机理。
影响因子：
15.3
作者：
Gu, Jiang;Gong, Encong;Zhang, Bo;Zheng, Jie;Gao, Zifen;Zhong, Yanfeng;Zou, Wanzhong;Zhan, Jun;Wang, Shenglan;Xie, Zhigang;Zhuang, Hui;Wu, Bingquan;Zhong, Haohao;Shao, Hongquan;Fang, Weigang;Gao, Dongshia;Pei, Fei;Li, Xingwang;He, Zhongpin;Xu, Danzhen;Shi, Xeying;Anderson, Virginia M;Leong, Anthony S-Y
通讯作者：
Leong, Anthony S-Y