Purification of biologically active simian virus 40 small tumor antigen.
Purification of biologically active simian virus 40 small tumor antigen.
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具有生物活性的猿猴病毒40小肿瘤抗原的纯化。
DOI：
10.1073/pnas.80.4.906
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发表时间：
1983
影响因子：
11.1
通讯作者：
Livingston,DM
中科院分区：
文献类型：
--
作者：
Bikel,I;Roberts,TM;Bladon,MT;Green,R;Amann,E;Livingston,DM
从杂交大肠杆菌trp-lac启动子和合适的核糖体结合位点下游克隆了猴病毒40小肿瘤抗原（t抗原）基因。由这种质粒（pTR865）转化的细菌克隆（865i）表达该基因，在最佳条件下，可以产生大于或等于其总蛋白的5%作为t抗原。这种克隆的可溶性提取物中t抗原相对较少，这在初始颗粒部分中发现。通过含尿素缓冲液的萃取，以显著纯化的形式从该部分中回收蛋白质。经凝胶过滤的t抗原富集溶液，得到高纯度的蛋白质。将该部分（不含尿素）或NaDodSO4凝胶纯化的865i t抗原（不含洗涤剂）注射到未转化的大鼠细胞中，观察到细胞内肌动蛋白电缆网络的溶解。
The simian virus 40 small tumor antigen (t antigen) gene has been cloned downstream from a hybrid Escherichia coli trp-lac promoter and a suitable ribosome binding site. A bacterial clone (865i) transformed by such a plasmid (pTR865) expresses this gene and, under optimal conditions, can produce greater than or equal to 5% of its total protein as t antigen. Soluble extracts of such a clone were relatively depleted in t antigen, which was found in the initial pellet fraction. The protein was recovered from this fraction in a significantly purified form by extraction with urea-containing buffer. After gel filtration of such t antigen-enriched solutions, highly purified protein was obtained. When either this fraction (freed of urea) or NaDodSO4 gel-purified 865i t antigen (rendered free of detergent) was injected into untransformed rat cells, dissolution of intracellular actin cable networks was observed.
维持转化状态需要 SV40 的大 T 和小 T 蛋白。
DOI：
10.1101/sqb.1980.044.01.037
发表时间：
1980
期刊：
Cold Spring Harbor symposia on quantitative biology
影响因子：
--
作者：
Frisque,RJ;Rifkin,DB;Topp,WC
通讯作者：
Topp,WC
SV40 F 基因蛋白的纯化和表征。
DOI：
10.1101/sqb.1980.044.01.018
发表时间：
1980
期刊：
Cold Spring Harbor symposia on quantitative biology
影响因子：
--
作者：
Tegtmeyer,P;Spillman,T;Schuetz,FR
通讯作者：
Schuetz,FR
T 抗原在 SV40 转化中的作用。
DOI：
10.1101/sqb.1980.044.01.036
发表时间：
1980
期刊：
Cold Spring Harbor symposia on quantitative biology
影响因子：
--
作者：
R. G. Martin;V. Setlow;A. Chepelinsky;R. Seif;A. Lewis;C. Scher;C. Stiles;J. Avila
通讯作者：
J. Avila