UBE3B and ZRANB1 polymorphisms and transcript abundance are associated with water holding capacity of porcine M. longissimus dorsi.
UBE3B and ZRANB1 polymorphisms and transcript abundance are associated with water holding capacity of porcine M. longissimus dorsi.
复制标题
UBE3B和ZRANB1多态性和转录本丰度与猪背最长肌持水能力相关
DOI：
10.1016/j.meatsci.2013.04.033
复制
发表时间：
2013
期刊：
影响因子：
7.1
通讯作者：
Wimmers K
中科院分区：
文献类型：
--
作者：
Huynh TP;Murani E;Maak S;Ponsuksili S;Wimmers K
在肉类成熟过程中，肌原纤维蛋白的降解会影响肉类的持水性。本研究试图确定编码泛素化相关蛋白的基因UBE3B和ZRANB1的多态性，并评估基因、转录本丰度和猪肉含水率之间的关系。在德国长白(GL，n=266)和皮特兰×(大白×德国长白)中，ZRANB1(C.552A>；G(p.Ile153Val))和UBE3B(c.1921A>；T和c.4292C>；T)两个沉默SNP与肌肉pH值、电导率、肉色或滴水损失相关(n=266)。此外，SNPs上的次要等位基因携带者往往会增加转录丰度。与UBE3B和ZRANB1分别对蛋白质降解的促进和抑制作用以及对WHC的预期影响一致，它们的表达与WHC呈正相关和负相关。结果表明，这两个基因中的SNPs与影响转录丰度和WHC的原因位点连锁。
The degradation of myofibrillar proteins during meat maturation affects the water holding capacity (WHC) of meat. Our study sought to identify polymorphisms in UBE3B and ZRANB1, genes encoding proteins involved in ubiquitination, and to evaluate the relationship between genotype, transcript abundance, and WHC of pork. A single SNP of ZRANB1, c.552A>G (p.Ile153Val), and two silent SNPs of UBE3B, c.1921A>T and c.4292C>T, were associated with muscle pH, conductivity, meat colour, or drip loss in German Landrace (GL, n=266) and Pietrain×(Large White×German Landrace) (PiF1, n=316). Further, carriers of the minor alleles at the SNPs tended to have increased transcript abundance. Consistent with the protein degradation promoting and inhibiting effects of UBE3B and ZRANB1, respectively, and the expected impact on WHC, their expressions were positively and negatively associated with WHC. The results implicate that the SNPs in both genes are in linkage with a causal site that affects transcript abundance and WHC.
登录
查看更多内容
大鼠红色和白色骨骼肌内泛素缀合物的定量和免疫细胞化学定位。
DOI：
10.1177/36.6.2835410
发表时间：
1988
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Riley,DA;Bain,JL;Ellis,S;Haas,AL
通讯作者：
Haas,AL
影响因子：
3.3
作者：
D. Attaix;L. Combaret;M. Pouch;D. Taillandier
通讯作者：
D. Attaix;L. Combaret;M. Pouch;D. Taillandier
DOI：
--
发表时间：
2000
期刊：
影响因子：
--
作者：
M. Sekikawa;K. Shimada;M. Fukushima;T. Ishikawa;J. Wakamatsu;M. Mikami
通讯作者：
M. Mikami
影响因子：
3.3
作者：
Darom A;Bening-Abu-Shach U;Broday L
通讯作者：
Broday L
死后牛肌肉中泛素结合蛋白磷酸酶抑制剂 1 的鉴定
影响因子：
8.8
作者：
P. Nyam;B. Jayawardana;J. A. Aro;K. Shimada;M. Fukushima;M. Sekikawa
通讯作者：
M. Sekikawa