Excess salt increases infarct size produced by photothrombotic distal middle cerebral artery occlusion in spontaneously hypertensive rats.
Excess salt increases infarct size produced by photothrombotic distal middle cerebral artery occlusion in spontaneously hypertensive rats.
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DOI：
10.1371/journal.pone.0097109
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Nabika T
中科院分区：
文献类型：
--
作者：
Yao H;Nabika T
众所周知，大脑循环很容易受到高盐负荷的影响。然而，目前还没有研究探讨过量盐分对局灶性脑缺血损伤的影响。自发性高血压大鼠(SHR)和血压正常的Wistar-京都大鼠(WKY)分别给予生理盐水或生理盐水14d后，制作光栓性大脑中动脉闭塞(MCAO)模型，48h后测定脑梗塞体积，同时测定SHR脑区白蛋白和血红蛋白含量。盐负荷对SHR和WKY的血压水平没有影响。MCAO后，盐负荷组局部脑血流量(CBF)明显低于对照组(P<0.05)。在SHR/IZM中，盐负荷组脑梗塞体积为112±27mm3，显著大于对照组的77±12mm3(p = 0.002)，而血脑屏障的破坏程度(脑白蛋白和血红蛋白水平)不受过量盐的影响。在WKY中，盐负荷并没有显著增加梗塞面积。这些结果表明盐负荷对高血压大鼠脑缺血后脑血流量和继发性脑梗塞的影响是不利的。
Cerebral circulation is known to be vulnerable to high salt loading. However, no study has investigated the effects of excess salt on focal ischemic brain injury. After 14 days of salt loading (0.9% saline) or water, spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto rats (WKY) were subjected to photothrombotic middle cerebral artery occlusion (MCAO), and infarct volume was determined at 48 h after MCAO: albumin and hemoglobin contents in discrete brain regions were also determined in SHR. Salt loading did not affect blood pressure levels in SHR and WKY. After MCAO, regional cerebral blood flow (CBF), determined with two ways of laser-Doppler flowmetry (one-point measurement or manual scanning), was more steeply decreased in the salt-loaded group than in the control group. In SHR/Izm, infarct volume in the salt-loaded group was 112±27 mm3, which was significantly larger than 77±12 mm3 in the control group (p = 0.002), while the extents of blood-brain barrier disruption (brain albumin and hemoglobin levels) were not affected by excess salt. In WKY, salt loading did not significantly increase infarct size. These results show the detrimental effects of salt loading on intra-ischemic CBF and subsequent brain infarction produced by phototrhombotic MCAO in hypertensive rats.
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