ROTUNDIFOLIA4 regulates cell proliferation along the body axis in Arabidopsis shoot.
ROTUNDIFOLIA4 regulates cell proliferation along the body axis in Arabidopsis shoot.
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DOI：
10.1093/pcp/pcq138
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发表时间：
2011-01
影响因子：
4.9
通讯作者：
Tsukaya H
中科院分区：
文献类型：
--
作者：
Ikeuchi M;Yamaguchi T;Kazama T;Ito T;Horiguchi G;Tsukaya H
分子遗传学已经成功地鉴定了叶大小调节因子，如转录因子、植物激素和信号分子。其中，拟南芥属的ROTUNDIFOLIA 4-LIKE/DEVIL（RTFL/DVL）家族是编码没有分泌信号序列的肽的基因，其独特之处在于它们的过表达子特异性地沿着近远轴具有减少的叶细胞数量。然而，由于RTFL/DVL与功能鉴定的结构域缺乏任何明显的同源性，并且由于RTFL/DVL之间的遗传冗余，它们的分子和发育作用尚不清楚。在这项研究中，我们专注于家族中的一个成员，ROTUNDIFOLIA 4（ROT 4），并确定了它的核心功能区域，我们发现在植物中没有蛋白水解加工。叶原基的发育分析表明，ROT 4过表达减少了叶片内的分生区的大小。此外，诱导的局部过表达表明，ROT 4作为一个调节器的叶片形状通过改变位置线索沿着纵轴。类似地，ROT 4过表达导致主花序茎的突出，再次表明位置线索沿着纵轴的变化。这些结果表明，ROT 4影响位置线索和细胞增殖沿着体轴。
Molecular genetics has been successful in identifying leaf- size regulators such as transcription factors, phytohormones, and signal molecules. Among them, a ROTUNDIFOLIA4-LIKE/DEVIL (RTFL/DVL) family of Arabidopsis, genes encoding peptides with no secretion-signal sequence, is unique in that their overexpressors have a reduced number of leaf cells specifically along the proximodistal axis. However, because the RTFL/DVL lack any obvious homology with functionally identified domains, and because of genetic redundancy among RTFL/DVL, their molecular and developmental roles are unclear. In this study we focused on one member in the family, ROTUNDIFOLIA4 (ROT4), and identified the core functional region within it and we found no proteolytic processing in planta. Developmental analysis of leaf primordia revealed that ROT4 overexpression reduces the meristematic zone size within the leaf blade. Moreover, induced local overexpression demonstrated that ROT4 acts as a regulator of the leaf shape via a change in positional cue along the longitudinal axis. Similarly, ROT4 overexpression results in a protrusion of the main inflorescence stem, again indicating a change in positional cue along the longitudinal axis. These results suggest that ROT4 affects the positional cue and cell proliferation along the body axis.
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影响因子：
7.2
作者：
Kim, GT;Fujioka, S;Tsukaya, H
通讯作者：
Tsukaya, H
影响因子：
7.2
作者：
Horiguchi, Gorou;Fujikura, Ushio;Tsukaya, Hirokazu
通讯作者：
Tsukaya, Hirokazu
影响因子：
0.7
作者：
Tsukaya, H
通讯作者：
Tsukaya, H
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
2.7
作者：
Donnelly, PM;Bonetta, D;Dengler, NG
通讯作者：
Dengler, NG