Genomic epidemiology of Mycobacterium bovis infection in sympatric badger and cattle populations in Northern Ireland.
Genomic epidemiology of Mycobacterium bovis infection in sympatric badger and cattle populations in Northern Ireland.
复制标题
DOI：
10.1099/mgen.0.001023
复制
发表时间：
2023-05
影响因子：
3.9
通讯作者：
Allen, Adrian R.
中科院分区：
文献类型：
--
作者：
Akhmetova, Assel;Guerrero, Jimena;McAdam, Paul;Salvador, Liliana C. M.;Crispell, Joseph;Lavery, John;Presho, Eleanor;Kao, Rowland R.;Biek, Roman;Menzies, Fraser;Trimble, Nigel;Harwood, Roland;Pepler, P. Theo;Oravcova, Katarina;Graham, Jordon;Skuce, Robin;du Plessis, Louis;Thompson, Suzan;Wright, Lorraine;Byrne, Andrew W.;Allen, Adrian R.
牛结核病（bTB）是一种流行病学上复杂的、多宿主的地方性疾病。对传播动态缺乏了解可能会破坏根除工作。病原体全基因组测序改善了流行病学推断，提供了一种手段来确定种间和种内宿主传播对疾病持续性的相对重要性。我们对619个异常数据集进行了测序， 牛分枝杆菌 来自北方爱尔兰100 km 2 bTB“热点”的獾和牛的分离株。历史分子分型数据允许针对地方性病原体谱系，其长期持续性提供了一个独特的机会，以无与伦比的细节研究疾病传播动力学。此外，为了评估獾种群遗传结构是否与病原体遗传多样性的空间分布相关，我们对该地区769只獾的毛发样本进行了微卫星基因分型。出生死亡模型和TransPhylo分析表明，牛可能是当地疫情的驱动力，从牛到獾的传播比獾到牛更常见。此外，景观中显著的獾种群遗传结构的存在与 M.牛 遗传多样性，这表明獾到獾的传播在传播动态中没有发挥主要作用。我们的数据与獾在传播中发挥的作用较小一致， M.牛 与牛相比，本研究中心的感染率。然而，我们假设，这个次要的作用可能仍然是重要的持久性。与其他地区的比较表明， M.牛 传播动态可能取决于环境，野生动物的作用难以概括。
Bovine tuberculosis (bTB) is a costly, epidemiologically complex, multi-host, endemic disease. Lack of understanding of transmission dynamics may undermine eradication efforts. Pathogen whole-genome sequencing improves epidemiological inferences, providing a means to determine the relative importance of inter- and intra-species host transmission for disease persistence. We sequenced an exceptional data set of 619 Mycobacterium bovis isolates from badgers and cattle in a 100 km2 bTB ‘hotspot’ in Northern Ireland. Historical molecular subtyping data permitted the targeting of an endemic pathogen lineage, whose long-term persistence provided a unique opportunity to study disease transmission dynamics in unparalleled detail. Additionally, to assess whether badger population genetic structure was associated with the spatial distribution of pathogen genetic diversity, we microsatellite genotyped hair samples from 769 badgers trapped in this area. Birth death models and TransPhylo analyses indicated that cattle were likely driving the local epidemic, with transmission from cattle to badgers being more common than badger to cattle. Furthermore, the presence of significant badger population genetic structure in the landscape was not associated with the spatial distribution of M. bovis genetic diversity, suggesting that badger-to-badger transmission is not playing a major role in transmission dynamics. Our data were consistent with badgers playing a smaller role in transmission of M. bovis infection in this study site, compared to cattle. We hypothesize, however, that this minor role may still be important for persistence. Comparison to other areas suggests that M. bovis transmission dynamics are likely to be context dependent, with the role of wildlife being difficult to generalize.
登录
查看更多内容
影响因子：
4.9
作者：
Biek R;Real LA
通讯作者：
Real LA
影响因子：
6.7
作者：
Biek, Roman;O'Hare, Anthony;Kao, Rowland R.
通讯作者：
Kao, Rowland R.
影响因子：
3.2
作者：
Allen AR;Skuce RA;Byrne AW
通讯作者：
Byrne AW
影响因子：
2.6
作者：
Chambers, Mark A.;Waterhouse, Sue;Hewinson, R. Glyn
通讯作者：
Hewinson, R. Glyn
影响因子：
3.8
作者：
Ciaravino, G.;Garcia-Saenz, A.;Napp, S.
通讯作者：
Napp, S.