Cell surface fluctuations regulate early embryonic lineage sorting.
Cell surface fluctuations regulate early embryonic lineage sorting.
复制标题
DOI：
10.1016/j.cell.2022.01.022
复制
发表时间：
2022-03-03
期刊：
影响因子：
64.5
通讯作者：
Chalut KJ
中科院分区：
文献类型：
--
作者：
Yanagida A;Corujo-Simon E;Revell CK;Sahu P;Stirparo GG;Aspalter IM;Winkel AK;Peters R;De Belly H;Cassani DAD;Achouri S;Blumenfeld R;Franze K;Hannezo E;Paluch EK;Nichols J;Chalut KJ
在发展过程中，谱系分离在时间和空间上是协调的。一个重要的例子是哺乳动物的内细胞群，其中原始内胚层（PrE，卵黄囊的建立者）与外胚层（EPI，胎儿的建立者）在物理上分离。虽然分子需求已经得到了很好的研究，但决定EPI和PrE空间分离的物理机制仍然难以捉摸。在此，我们研究了EPI和预分选的力学基础。我们发现，与经典分选模型中静态细胞表面机械参数的差异不同，表面波动的差异有力地确保了物理谱系分选。这些不同的表面波动与不同的细胞流动性系统相关，我们认为它们共同构成了EPI和PrE谱系的非平衡分选机制。通过实验和建模相结合，我们确定细胞表面动力学是协调创始胚胎谱系正确空间分离的关键因素。分离不能用经典的静态力学模型来解释，而是用一个动态参数——细胞表面波动来解释。波动与细胞流动性有关，共同构成一个非平衡分选模型。在早期哺乳动物胚胎中，细胞表面的差异波动，而不是静态的物理参数，解释了内细胞团中原始内胚层和外胚层的分选。
In development, lineage segregation is coordinated in time and space. An important example is the mammalian inner cell mass, in which the primitive endoderm (PrE, founder of the yolk sac) physically segregates from the epiblast (EPI, founder of the fetus). While the molecular requirements have been well studied, the physical mechanisms determining spatial segregation between EPI and PrE remain elusive. Here, we investigate the mechanical basis of EPI and PrE sorting. We find that rather than the differences in static cell surface mechanical parameters as in classical sorting models, it is the differences in surface fluctuations that robustly ensure physical lineage sorting. These differential surface fluctuations systematically correlate with differential cellular fluidity, which we propose together constitute a non-equilibrium sorting mechanism for EPI and PrE lineages. By combining experiments and modeling, we identify cell surface dynamics as a key factor orchestrating the correct spatial segregation of the founder embryonic lineages. EPI and PrE mechanically segregate in early mouse embryos Segregation is not explained by classical, static mechanical models Segregation is instead explained by a dynamic parameter, cell surface fluctuations Fluctuations link to cell fluidity, together comprising a non-equilibrium sorting model In early mammalian embryos differential cell surface fluctuations, rather than static physical parameters, account for the sorting of the primitive endoderm from the epiblast in the inner cell mass.
登录
查看更多内容
影响因子：
4
作者：
Chugh P;Paluch EK
通讯作者：
Paluch EK
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
21.3
作者：
Boroviak T;Loos R;Bertone P;Smith A;Nichols J
通讯作者：
Nichols J
影响因子：
5.2
作者：
Artus, Jerome;Kang, Minjung;Cohen-Tannoudji, Michel;Hadjantonakis, Anna-Katerina
通讯作者：
Hadjantonakis, Anna-Katerina
影响因子：
11.8
作者：
Chazaud, Claire;Yamanaka, Yojiro;Rossant, Janet
通讯作者：
Rossant, Janet