Comparison of Mitochondrial Reactive Oxygen Species Production of Ectothermic and Endothermic Fish Muscle.
Comparison of Mitochondrial Reactive Oxygen Species Production of Ectothermic and Endothermic Fish Muscle.
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DOI：
10.3389/fphys.2017.00704
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发表时间：
2017
影响因子：
4
通讯作者：
Treberg JR
中科院分区：
文献类型：
--
作者：
Wiens L;Banh S;Sotiri E;Jastroch M;Block BA;Brand MD;Treberg JR
最近，我们证明了分离的肌肉线粒体产生活性氧的能力，测量为H2O2流出，是温度敏感的在分离的肌肉线粒体的外温鱼类和大鼠，一个代表性的吸热哺乳动物。然而，在生理温度下（鱼和大鼠分别为15° C和37°C），大鼠中产生H2O2的总线粒体电子通量的分数（FEL）远低于鱼。这些结果表明，与恒温相关的体温升高可能导致线粒体ROS产生相对于呼吸能力的代偿性减少。为了验证这一假设，我们比较慢颤（红色）肌肉线粒体从吸热的太平洋蓝鳍金枪鱼（东方金枪鱼）与线粒体从三个变温鱼类[虹鳟鱼（虹鳟鱼mykiss），鲤鱼（鲤鱼），和湖鲟鱼（鲟）]和大鼠。在一个共同的测定温度（25°C）的线粒体呼吸和H2O2流出率是相似的金枪鱼和其他鱼类。无论是内温型还是外温型，鱼类线粒体的热敏感性都是相似的。在相同温度下比较金枪鱼和大鼠，呼吸速率相似，或更低，取决于线粒体底物。在常见的测定温度（25°C）下，鱼类的FEL没有差异，但鱼类的FEL明显高于大鼠。总体而言，吸热和变暖的太平洋蓝鳍金枪鱼红肌肉可能会增加肌肉线粒体的ROS生产的潜力，但在这个物种的吸热的演变不一定与ROS生产相对于线粒体的呼吸能力的补偿性减少。
Recently we demonstrated that the capacity of isolated muscle mitochondria to produce reactive oxygen species, measured as H2O2 efflux, is temperature-sensitive in isolated muscle mitochondria of ectothermic fish and the rat, a representative endothermic mammal. However, at physiological temperatures (15° and 37°C for the fish and rat, respectively), the fraction of total mitochondrial electron flux that generated H2O2, the fractional electron leak (FEL), was far lower in the rat than in fish. Those results suggested that the elevated body temperatures associated with endothermy may lead to a compensatory decrease in mitochondrial ROS production relative to respiratory capacity. To test this hypothesis we compare slow twitch (red) muscle mitochondria from the endothermic Pacific bluefin tuna (Thunnus orientalis) with mitochondria from three ectothermic fishes [rainbow trout (Oncorhynchus mykiss), common carp (Cyprinus carpio), and the lake sturgeon (Acipenser fulvescens)] and the rat. At a common assay temperature (25°C) rates of mitochondrial respiration and H2O2 efflux were similar in tuna and the other fishes. The thermal sensitivity of fish mitochondria was similar irrespective of ectothermy or endothermy. Comparing tuna to the rat at a common temperature, respiration rates were similar, or lower depending on mitochondrial substrates. FEL was not different across fish species at a common assay temperature (25°C) but was markedly higher in fishes than in rat. Overall, endothermy and warming of Pacific Bluefin tuna red muscle may increase the potential for ROS production by muscle mitochondria but the evolution of endothermy in this species is not necessarily associated with a compensatory reduction of ROS production relative to the respiratory capacity of mitochondria.
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影响因子：
2.8
作者：
Clark, T. D.;Brandt, W. T.;Block, B. A.
通讯作者：
Block, B. A.
DOI：
10.1007/bf02338293
发表时间：
1996-12-01
期刊：
JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY
影响因子：
--
作者：
Lauff, RF;Wood, CH
通讯作者：
Wood, CH
影响因子：
6.4
作者：
Jastroch M;Divakaruni AS;Mookerjee S;Treberg JR;Brand MD
通讯作者：
Brand MD
影响因子：
1.6
作者：
Dickson, KA;Graham, JB
通讯作者：
Graham, JB
影响因子：
4.1
作者：
Boustany, Andre M.;Matteson, Robyn;Block, Barbara A.
通讯作者：
Block, Barbara A.