Comparison of physicochemical characteristics and anticoagulant activities of polysaccharides from three sea cucumbers.
Comparison of physicochemical characteristics and anticoagulant activities of polysaccharides from three sea cucumbers.
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三种海参多糖理化特性及抗凝血活性比较
DOI：
10.3390/md11020399
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发表时间：
2013-02-05
期刊：
影响因子：
5.4
通讯作者：
Zhao J
中科院分区：
文献类型：
--
作者：
Luo L;Wu M;Xu L;Lian W;Xiang J;Lu F;Gao N;Xiao C;Wang S;Zhao J
为了在不同的无脊椎动物结缔组织中寻找硫酸多糖并检测其生物活性，我们从三种海参的体壁中分离得到了三种类型的多糖。对它们的理化性质和抗凝血活性进行了检测和比较。化学成分分析和核磁共振分析表明，三种藻类均含有两种类型的多糖--岩藻多糖和岩藻糖化硫酸软骨素(FuCS)，此外还发现一种中性多糖。中性α-葡聚糖首次从海参中分离得到。不同种类海参的同种多糖具有相似的理化性质和抗凝血活性，但不同类型的多糖具有明显的差异，这可能是由于其单糖组成、电荷和平均分子质量不同所致。FuCSs的抗凝血活性强于硫酸岩藻聚糖，尽管FuCSs的分子尺寸比硫酸岩藻聚糖小，而中性葡聚糖则没有活性，正如在没有硫酸盐的情况下预期的那样。因此，不同类型的多糖的抗凝血活性可能与单糖组成和硫酸盐含量有关。初步分析表明，FuCSs的硫酸盐化模式可能是导致抗凝血活性差异的原因。我们的数据有助于阐明海参多糖的构效关系。
In order to search for sulfated polysaccharides in different invertebrate connective tissues and to examine their biological activities, we have isolated three types of polysaccharides from the body wall of the three sea cucumbers Holothuria edulis, Apostichopus japonicas and Holothuria nobilis. The physicochemical properties and anticoagulant activities of these polysaccharides were examined and compared. The chemical composition analysis and nuclear magnetic resonance (NMR) analysis indicate that two types of polysaccharides, sulfated fucan and fucosylated chondroitin sulfate (FuCS), were found in all of the three species and in addition a neutral glycan was observed in H. edulis. The neutral α-glucan was firstly obtained from sea cucumber. The same type of polysaccharides from different species of sea cucumbers have similar physicochemical properties and anticoagulant activities, but those of different types of glycans are significantly different, possibly due to their different monosaccharide compositions, electric charges and average molecular weights. The FuCSs have stronger anticoagulant activities than the sulfated fucans, although the molecular sizes of the FuCSs are lower than those of the sulfated fucans, whereas the neutral glucan has no activity, as expected from the absence of sulfate. Thus, anticoagulant activities of the different type of polysaccharides are likely to relate to monosaccharide composition and sulfate content. Preliminary analysis suggests that the sulfation patterns of the FuCSs may result in the difference in anticoagulant activities. Our data could help elucidate the structure-activity relationship of the sea cucumber polysaccharides.
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影响因子：
4.8
作者：
Mourao, PAS;Pereira, MS;Abildgaard, U
通讯作者：
Abildgaard, U
影响因子：
5.4
作者：
Gao N;Wu M;Liu S;Lian W;Li Z;Zhao J
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影响因子：
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作者：
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11.2
作者：
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影响因子：
2.9
作者：
Fu, DT;Zopf, D
通讯作者：
Zopf, D