Metabolite profiling and network analysis reveal coordinated changes in grapevine water stress response.
Metabolite profiling and network analysis reveal coordinated changes in grapevine water stress response.
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DOI：
10.1186/1471-2229-13-184
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发表时间：
2013-11-20
影响因子：
5.3
通讯作者：
Fait A
中科院分区：
文献类型：
--
作者：
Hochberg U;Degu A;Toubiana D;Gendler T;Nikoloski Z;Rachmilevitch S;Fait A
研究了设拉子和赤霞珠两个葡萄品种对水分亏缺的反应，这两个品种具有不同的水力行为(Hochberg等人)。物理。种。147：443-453,2012)。渐进性水分亏缺会影响叶片水势的变化，并伴随着代谢变化。在两个品种中，但设拉子品种的水分亏缺比赤霞珠品种更严重，水分亏缺引起了向高渗透压和低C/N比的转变，后者还反映在氨基酸如Pro、Val、Leu、Thr和Trp的显著增加，大多数有机酸的减少，以及苯丙烷途径的改变。主成分分析表明，初级代谢的变化主要与水分胁迫有关，而专化性代谢的多样化主要与品种有关。在韧皮部汁液中，干旱的特征是ABA浓度较高，苯甲酸水平的主要变化与气孔导度降低和维管束的柔性化相一致。设拉子树液中的羟基苯甲酸衍生物丰度较高，反映了其生物合成能力的增强。基于相关性的网络分析表明，与赤霞珠相比，设拉子有相当大的和高度协调的与胁迫相关的变化，反映在其在胁迫下代谢网络连接性的增强。网络分析还强调了与胁迫相关的主要代谢物，如Pro、栎素和抗坏血酸的结构作用，这些物质在缺水情况下极大地改变了它们在设拉子网络中的连通性。综上所述，结果表明，不同葡萄品种对水分亏缺具有共同的代谢反应。中枢代谢，特别是氮代谢，在葡萄的逆境反应中起着重要作用。在品种水平上，赤霞珠具有较温和的代谢扰动，这可能是由于胁迫诱导时气孔调节较紧密所致。网络分析被成功地应用于表征植物逆境分子反应和鉴定在藤本植物逆境反应中具有重要结构和生物学作用的代谢物。
Grapevine metabolism in response to water deficit was studied in two cultivars, Shiraz and Cabernet Sauvignon, which were shown to have different hydraulic behaviors (Hochberg et al. Physiol. Plant. 147:443–453, 2012). Progressive water deficit was found to effect changes in leaf water potentials accompanied by metabolic changes. In both cultivars, but more intensively in Shiraz than Cabernet Sauvignon, water deficit caused a shift to higher osmolality and lower C/N ratios, the latter of which was also reflected in marked increases in amino acids, e.g., Pro, Val, Leu, Thr and Trp, reductions of most organic acids, and changes in the phenylpropanoid pathway. PCA analysis showed that changes in primary metabolism were mostly associated with water stress, while diversification of specialized metabolism was mostly linked to the cultivars. In the phloem sap, drought was characterized by higher ABA concentration and major changes in benzoate levels coinciding with lower stomatal conductance and suberinization of vascular bundles. Enhanced suberin biosynthesis in Shiraz was reflected by the higher abundance of sap hydroxybenzoate derivatives. Correlation-based network analysis revealed that compared to Cabernet Sauvignon, Shiraz had considerably larger and highly coordinated stress-related changes, reflected in its increased metabolic network connectivity under stress. Network analysis also highlighted the structural role of major stress related metabolites, e.g., Pro, quercetin and ascorbate, which drastically altered their connectedness in the Shiraz network under water deficit. Taken together, the results showed that Vitis vinifera cultivars possess a common metabolic response to water deficit. Central metabolism, and specifically N metabolism, plays a significant role in stress response in vine. At the cultivar level, Cabernet Sauvignon was characterized by milder metabolic perturbations, likely due to a tighter regulation of stomata upon stress induction. Network analysis was successfully implemented to characterize plant stress molecular response and to identify metabolites with a significant structural and biological role in vine stress response.
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影响因子：
5.3
作者：
Cramer GR;Urano K;Delrot S;Pezzotti M;Shinozaki K
通讯作者：
Shinozaki K
影响因子：
5.3
作者：
Cramer GR;Van Sluyter SC;Hopper DW;Pascovici D;Keighley T;Haynes PA
通讯作者：
Haynes PA
DOI：
10.1071/pp99019
发表时间：
1999-01-01
期刊：
AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY
影响因子：
--
作者：
Escalona, JM;Flexas, J;Medrano, H
通讯作者：
Medrano, H
影响因子：
7.4
作者：
Ferrario-Méry, S;Valadier, MH;Foyer, CH
通讯作者：
Foyer, CH
影响因子：
5.1
作者：
Boss, PK;Davies, C;Robinson, SP
通讯作者：
Robinson, SP