Proteomic analysis indicates massive changes in metabolism prior to the inhibition of growth and photosynthesis of grapevine (Vitis vinifera L.) in response to water deficit.
Proteomic analysis indicates massive changes in metabolism prior to the inhibition of growth and photosynthesis of grapevine (Vitis vinifera L.) in response to water deficit.
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DOI：
10.1186/1471-2229-13-49
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发表时间：
2013-03-21
影响因子：
5.3
通讯作者：
Haynes PA
中科院分区：
文献类型：
--
作者：
Cramer GR;Van Sluyter SC;Hopper DW;Pascovici D;Keighley T;Haynes PA
赤霞珠葡萄藤在 16 天内经历了逐渐增加的缺水状况。测量芽伸长和光合作用以了解对缺水的生理反应。使用纳流液相色谱-串联质谱法 (nanoLC-MS/MS) 分析了随着时间的推移水分亏缺对生长中的茎尖（包括四个未成熟的叶子）中单个蛋白质丰度的影响。第 4 天后，水分亏缺逐渐降低枝条伸长、气孔导度和光合作用；通过鸟枪法蛋白质组学鉴定了 2277 种蛋白质，所有蛋白质的蛋白质丰度的平均 CV 为 9%。在所有样本中发现的 942 种蛋白质中有 472 种 (50%) 受到缺水的显着影响。 472 种蛋白质分为四组：早期和晚期响应蛋白质谱的丰度增加和减少。藤蔓很早就感觉到缺水，似乎已经适应了压力，因为许多蛋白质的丰度在枝条伸长、气孔导度和光合作用下降之前发生了变化。早期响应蛋白的主要功能类别包括光合作用、糖酵解、翻译、抗氧化防御和生长相关类别（类固醇代谢和水运输），而晚期响应蛋白的其他蛋白质主要涉及运输、光呼吸、抗氧化剂、氨基酸和碳水化合物代谢。蛋白质组对缺水的反应是动态的，涉及翻译、能量、抗氧化防御和类固醇代谢的蛋白质丰度发生了早期的显着变化。这些蛋白质的丰度在芽伸长、气孔导度或光合作用出现任何可检测到的下降之前发生变化。已知许多早期反应蛋白受到转录后修饰（例如磷酸化）的调节。蛋白质组学分析表明，植物代谢发生了巨大而实质性的变化，这些变化似乎在植物对水分亏缺做出任何重大损伤之前反应的早期阶段，将碳和能量输送到抗氧化防御中。
Cabernet Sauvignon grapevines were exposed to a progressive, increasing water defict over 16 days. Shoot elongation and photosynthesis were measured for physiological responses to water deficit. The effect of water deficit over time on the abundance of individual proteins in growing shoot tips (including four immature leaves) was analyzed using nanoflow liquid chromatography - tandem mass spectrometry (nanoLC-MS/MS). Water deficit progressively decreased shoot elongation, stomatal conductance and photosynthesis after Day 4; 2277 proteins were identified by shotgun proteomics with an average CV of 9% for the protein abundance of all proteins. There were 472 out of 942 (50%) proteins found in all samples that were significantly affected by water deficit. The 472 proteins were clustered into four groups: increased and decreased abundance of early- and late-responding protein profiles. Vines sensed the water deficit early, appearing to acclimate to stress, because the abundance of many proteins changed before decreases in shoot elongation, stomatal conductance and photosynthesis. Predominant functional categories of the early-responding proteins included photosynthesis, glycolysis, translation, antioxidant defense and growth-related categories (steroid metabolism and water transport), whereas additional proteins for late-responding proteins were largely involved with transport, photorespiration, antioxidants, amino acid and carbohydrate metabolism. Proteomic responses to water deficit were dynamic with early, significant changes in abundance of proteins involved in translation, energy, antioxidant defense and steroid metabolism. The abundance of these proteins changed prior to any detectable decreases in shoot elongation, stomatal conductance or photosynthesis. Many of these early-responding proteins are known to be regulated by post-transcriptional modifications such as phosphorylation. The proteomics analysis indicates massive and substantial changes in plant metabolism that appear to funnel carbon and energy into antioxidant defenses in the very early stages of plant response to water deficit before any significant injury.
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影响因子：
5.3
作者：
Cramer GR;Urano K;Delrot S;Pezzotti M;Shinozaki K
通讯作者：
Shinozaki K
影响因子：
7.3
作者：
Alvarez, Sophie;Marsh, Ellen L.;Schachtman, Daniel P.
通讯作者：
Schachtman, Daniel P.
影响因子：
10.7
作者：
Goremykin, Vadim V.;Salamini, Francesco;Viola, Roberto
通讯作者：
Viola, Roberto
DOI：
10.1007/s11101-011-9211-7
发表时间：
2011-09
期刊：
Phytochemistry reviews : proceedings of the Phytochemical Society of Europe
影响因子：
--
作者：
Dao TT;Linthorst HJ;Verpoorte R
通讯作者：
Verpoorte R
影响因子：
3.7
作者：
Benešová M;Holá D;Fischer L;Jedelský PL;Hnilička F;Wilhelmová N;Rothová O;Kočová M;Procházková D;Honnerová J;Fridrichová L;Hniličková H
通讯作者：
Hniličková H