Involvement of Integrin αvβ3 in Cell Adhesion, Motility, and Liver Metastasis of Murine RAW117 Large Cell Lymphoma
Involvement of Integrin αvβ3 in Cell Adhesion, Motility, and Liver Metastasis of Murine RAW117 Large Cell Lymphoma
复制标题
整合素αvβ3参与小鼠RAW117大细胞淋巴瘤的细胞粘附、运动和肝转移
DOI：
--
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
G. Nicolson
中科院分区：
文献类型：
--
作者：
Z. Yun;D. Menter;G. Nicolson
Abstract The molecules that mediate metastatic cell homing to specific organ sites remain largely unidentified. As a target organ for metastasis, the liver is a unique environment characterized by fenestrated sinusoidal endothelium, lack of a complete basement membrane, and production of serum components, including fibronectin and vitronectin. We examined a series of murine RAW117 large cell lymphoma variants selected in vivo for liver-colonizing properties (H10 ≫ L17 > P). Compared with L17 or P cells, the highly liver-colonizing H10 cells expressed much higher levels of surface integrin αvβ3, as shown by affinity chromatography, immunoprecipitation, and flow cytometry. H10 cells adhered at higher rates to vitronectin and fibronectin than to fibrinogen, fibrin, laminin, and type I collagen. Among the RGD peptides, H10 cells adhered at significantly higher rates to the polymeric RGD peptide (glycyl-arginyl-glycyl-aspartyl-serine)4 than to monomeric RGD peptides. H10 cells were able to spread on immobilized vitronectin with highly polarized morphology but not on fibronectin. In contrast, the poorly liver-metastatic P and L17 cells did not adhere or spread well on vitronectin or fibronectin. H10 cells also migrated toward vitronectin concentration gradients. Blocking cell surface αvβ3 molecules with specific anti-β3 monoclonal antibodies resulted in significant decreases in the adhesion of H10 cells to vitronectin and (glycyl-arginyl-glycyl-aspartyl-serine)4 and significant inhibition of the formation of experimental liver metastases. These data suggest an important role of integrin αvβ3 in the metastasis of RAW117 cells to the liver.
登录
查看更多内容
影响因子：
11.2
作者：
S. Albelda;S. Mette;D. Elder;R. Stewart;L. Damjanovich;M. Herlyn;C. Buck
通讯作者：
S. Albelda;S. Mette;D. Elder;R. Stewart;L. Damjanovich;M. Herlyn;C. Buck
大鼠肝脏内单核细胞分泌特异性 IgA。
影响因子：
--
作者：
Altorfer,J;Hardesty,SJ;Jones,AL
通讯作者：
Jones,AL
鼠大细胞淋巴瘤选定转移变体的粘附、侵袭和生长特性。
DOI：
--
发表时间：
1989
期刊：
Invasion & metastasis
影响因子：
--
作者：
Nicolson,GL;Belloni,PN;Tressler,RJ;Dulski,K;Inoue,T;Cavanaugh,PG
通讯作者：
Cavanaugh,PG
小鼠肿瘤细胞中 α IIb β 3 整合素的鉴定。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Chen,YQ;Gao,X;Timar,J;Tang,D;Grossi,IM;Chelladurai,M;Kunicki,TJ;Fligiel,SE;Taylor,JD;Honn,KV
通讯作者：
Honn,KV