Promoter-level transcriptome in primary lesions of endometrial cancer identified biomarkers associated with lymph node metastasis.
Promoter-level transcriptome in primary lesions of endometrial cancer identified biomarkers associated with lymph node metastasis.
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DOI：
10.1038/s41598-017-14418-5
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发表时间：
2017-10-26
影响因子：
4.6
通讯作者：
Kawaji H
中科院分区：
文献类型：
--
作者：
Yoshida E;Terao Y;Hayashi N;Mogushi K;Arakawa A;Tanaka Y;Ito Y;Ohmiya H;Hayashizaki Y;Takeda S;Itoh M;Kawaji H
对于子宫内膜癌患者，建议进行淋巴结切除术以排除很少转移的癌细胞。即使在复发风险较低的患者中也可以进行该手术，尽管存在诸如水肿等并发症的风险。因此，在淋巴结切除术前准确识别无淋巴结转移（LN-）病例的方法是非常必要的。我们通过用CAGE（Cap Analysis Gene Expression）检测子宫内膜癌的原发性病变来解决这个临床问题，CAGE可以量化基因组中启动子水平的表达。14个图谱描绘了LN +和LN-病例之间不同的转录网络，这些病例被归类为具有低或中等复发风险。随后对115例原发性肿瘤进行的定量逆转录聚合酶链反应（qRT-PCR）分析显示，SEMA 3D mRNA和TACC 2亚型通过新型启动子表达，作为有前景的生物标志物，在联合使用时具有高准确度（受试者工作特征曲线下的面积，0.929）。我们的高分辨率转录组提供了子宫内膜癌LN + /LN-状态的不同分子特征的证据，提高了术前诊断的可能性，以减少患者不必要的手术，使复发风险最小。
For endometrial cancer patients, lymphadenectomy is recommended to exclude rarely metastasized cancer cells. This procedure is performed even in patients with low risk of recurrence despite the risk of complications such as lymphedema. A method to accurately identify cases with no lymph node metastases (LN−) before lymphadenectomy is therefore highly required. We approached this clinical problem by examining primary lesions of endometrial cancers with CAGE (Cap Analysis Gene Expression), which quantifies promoter-level expression across the genome. Fourteen profiles delineated distinct transcriptional networks between LN + and LN− cases, within those classified as having the low or intermediate risk of recurrence. Subsequent quantitative reverse transcription polymerase chain reaction (qRT-PCR) analyses of 115 primary tumors showed SEMA3D mRNA and TACC2 isoforms expressed through a novel promoter as promising biomarkers with high accuracy (area under the receiver operating characteristic curve, 0.929) when used in combination. Our high-resolution transcriptome provided evidence of distinct molecular profiles underlying LN + /LN− status in endometrial cancers, raising the possibility of preoperative diagnosis to reduce unnecessary operations in patients with minimum recurrence risk.
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影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1097/pas.0b013e318299f2ab
发表时间：
2013-11
期刊：
The American journal of surgical pathology
影响因子：
--
作者：
Euscher E;Fox P;Bassett R;Al-Ghawi H;Ali-Fehmi R;Barbuto D;Djordjevic B;Frauenhoffer E;Kim I;Hong SR;Montiel D;Moschiano E;Roma A;Silva E;Malpica A
通讯作者：
Malpica A
影响因子：
7
作者：
Harrow J;Frankish A;Gonzalez JM;Tapanari E;Diekhans M;Kokocinski F;Aken BL;Barrell D;Zadissa A;Searle S;Barnes I;Bignell A;Boychenko V;Hunt T;Kay M;Mukherjee G;Rajan J;Despacio-Reyes G;Saunders G;Steward C;Harte R;Lin M;Howald C;Tanzer A;Derrien T;Chrast J;Walters N;Balasubramanian S;Pei B;Tress M;Rodriguez JM;Ezkurdia I;van Baren J;Brent M;Haussler D;Kellis M;Valencia A;Reymond A;Gerstein M;Guigó R;Hubbard TJ
通讯作者：
Hubbard TJ
DOI：
10.1002/path.4847
发表时间：
2017-02
期刊：
The Journal of pathology
影响因子：
--
作者：
Heng YJ;Lester SC;Tse GM;Factor RE;Allison KH;Collins LC;Chen YY;Jensen KC;Johnson NB;Jeong JC;Punjabi R;Shin SJ;Singh K;Krings G;Eberhard DA;Tan PH;Korski K;Waldman FM;Gutman DA;Sanders M;Reis-Filho JS;Flanagan SR;Gendoo DM;Chen GM;Haibe-Kains B;Ciriello G;Hoadley KA;Perou CM;Beck AH
通讯作者：
Beck AH
影响因子：
6.4
作者：
Hoang, Lien N.;Han, Guangming;Lee, Cheng-Han
通讯作者：
Lee, Cheng-Han