Study on the Mechanism of MicroRNA551b-5p in Severe Acute Pancreatitis Capillary Leakage Syndrome.
Study on the Mechanism of MicroRNA551b-5p in Severe Acute Pancreatitis Capillary Leakage Syndrome.
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MicroRNA551b-5p在重症急性胰腺炎毛细血管渗漏综合征中的作用机制研究
DOI：
10.1155/2022/6373757
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Liu P
中科院分区：
文献类型：
--
作者：
Yao Q;Ke HJ;Yang Q;Liao GY;Liu P
目的探讨microRNA551b-5p（miR-551b-5p）在重症急性胰腺炎发病中的作用。方法采用实时荧光定量聚合酶链反应（qPCR）技术检测miR-551b-5p在分化的人脐静脉内皮细胞（HUVECs）中的表达。此外，在HUVECs Transwell试验中观察了异常表达的miR-551b-5p的影响。采用Western blot、FITC-鬼笔环肽和免疫荧光染色检测重症急性胰腺炎毛细血管渗漏综合征相关蛋白的表达。最后，对影响miR-551b-5p表达的相关因素、靶基因及其作用进行了分析。结果miR-551b-5p过表达可明显促进EGFR、AKT3和AQP5的表达，而抑制JAM3、AQP1和occludin的表达。在功能上，miR-551b-5p过表达的细胞骨架相对松散，具有明显的空泡，并且miR-551b-5p过表达增加了HUVECs的通透性。结论miR-551b-5p过表达通过上调EGFR/AKT3通路，下调occludin和JAM3表达，促进血管内皮通透性的改变。
Objective This study focused on investigating the effects of microRNA551b-5p (miR-551b-5p) on severe acute pancreatitis. Methods Initially, quantitative real-time polymerase chain reaction (qPCR) is employed to determine the expression of miR-551b-5p in differentiated human umbilical vein endothelial cells (HUVECs). Further, the effects of aberrantly expressed miR-551b-5p in HUVECs Transwell assay. The expressions of proteins associated with severe acute pancreatitis capillary leakage syndrome are determined by Western blot, FITC-phalloidin, and immunofluorescence stainings. Finally, the correlative factor and the target genes of miR-551b-5p, as well as their contributions, are assessed. Results We observed that overexpression of miR-551b-5p distinctly promoted the expression of EGFR, AKT3, and AQP5, while it suppressed the expression of JAM3, AQP1, and occludin. Functionally, the cytoskeleton of the miR-551b-5p overexpression was relatively loose with apparent vacuoles, and overexpression of miR-551b-5p increased the permeability of HUVECs. Conclusion miR-551b-5p overexpression promoted changes in vascular endothelial permeability via upregulation of the EGFR/AKT3 pathway and downregulation of occludin and JAM3.
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