Dual simulated childbirth injuries result in slowed recovery of pudendal nerve and urethral function.
Dual simulated childbirth injuries result in slowed recovery of pudendal nerve and urethral function.
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DOI：
10.1002/nau.20632
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发表时间：
2009
影响因子：
2
通讯作者：
Damaser, Margot
中科院分区：
文献类型：
--
作者：
Jiang, Hai-Hong;Pan, Hui Q.;Gustilo-Ashby, Marcus A.;Gill, Bradley;Glaab, Jonathan;Zaszczurynski, Paul;Damaser, Margot
关键词：
骨盆底肌肉创伤和阴部神经损伤与分娩后压力性尿失禁（SUI）的发生有关。在这项研究中，我们调查了这些损伤的组合如何影响恢复。67只雌性Sprague-Dawley大鼠接受阴道扩张（VD）、阴部神经挤压（PNC）、PNC和VD（PNC+VD）、阴部神经切断（PNT）或作为未操作的对照。分别于伤后4d、3周、6周在乌拉坦麻醉下记录阴部神经运动分支电位（PNMBP）、尿道外括约肌肌电图（EUS EMG）和经尿道膀胱测压。确定泄漏点压力（LPP）测试期间是否存在保护反射（PNMBP或EUS EMG活动的频率和振幅增加）。对照组始终表现出保护反射。VD后4 d EUS EMG活动消失，PNMBP活动反映了保护反射，3周后EUS EMG活动恢复。PNC后4天，EUS EMG和PNMBP活性均被消除，但在3周时表现出显著恢复。PNC+VD后4天EUS肌电图和神经活动均消失，3周后几乎没有恢复，6周后保护反射明显恢复。在PNT后的所有时间点观察到几乎没有恢复。LPP结果反映了EUS EMG活动的减少。PNC+VD后的功能恢复比单独PNC或VD后慢。未来的工作将旨在测试方法，以促进神经再生和恢复后，这种临床相关的双重损伤。
Pelvic floor muscle trauma and pudendal nerve injury have been implicated in stress urinary incontinence (SUI) development after childbirth. In this study, we investigated how combinations of these injuries affect recovery. Sixty-seven female Sprague-Dawley rats underwent vaginal distension (VD), pudendal nerve crush (PNC), PNC and VD (PNC+VD), pudendal nerve transection (PNT), or served as unmanipulated controls. Four days, 3 weeks, or 6 weeks after injury, we simultaneously recorded pudendal nerve motor branch potentials (PNMBP), external urethral sphincter electromyography (EUS EMG), and transurethral bladder pressure under urethane anesthesia. The presence of a guarding reflex (increased frequency & amplitude of PNMBP or EUS EMG activity) during leak point pressure (LPP) testing was determined. Controls consistently demonstrated a guarding reflex. Four days after VD, EUS EMG activity was eliminated, but PNMBP activity reflected the guarding reflex; EUS EMG activity recovered after 3 weeks. Four days after PNC, both EUS EMG and PNMBP activity were eliminated, but demonstrated significant recovery at 3 weeks. Four days after PNC+VD both EUS EMG and nerve activity were eliminated, and little recovery was observed after 3 weeks with significant recovery of the guarding reflex 6 weeks after injury. Little recovery was observed at all time points after PNT. LPP results mirrored the reduction in EUS EMG activity. Functional recovery occurs more slowly after PNC+VD than after either PNC or VD alone. Future work will be aimed at testing methods to facilitate neuroregeneration and recovery after this clinically relevant dual injury.
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影响因子：
2.9
作者：
PACHECO, P;MARTINEZGOMEZ, M;KOMISARUK, BR
通讯作者：
KOMISARUK, BR
DOI：
10.1152/ajprenal.00176.2007
发表时间：
2007-11-01
影响因子：
4.2
作者：
Damaser, Margot S.;Samplaski, Mary K.;Kerns, James M.
通讯作者：
Kerns, James M.
DOI：
10.1196/annals.1389.034
发表时间：
2007-01-01
期刊：
REPRODUCTIVE BIOMECHANICS
影响因子：
--
作者：
Ashton-Miller, James A.;Delancey, John O. L.
通讯作者：
Delancey, John O. L.
DOI：
10.1152/ajpregu.00784.2006
发表时间：
2007-04-01
影响因子：
2.8
作者：
Pan, H. Q.;Kerns, J. M.;Damaser, M. S.
通讯作者：
Damaser, M. S.
影响因子：
9.6
作者：
SNOOKS, SJ;SWASH, M;HENRY, MM
通讯作者：
HENRY, MM