Global distribution of a chlorophyll f cyanobacterial marker.
Global distribution of a chlorophyll f cyanobacterial marker.
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DOI：
10.1038/s41396-020-0670-y
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发表时间：
2020-09
期刊：
影响因子：
--
通讯作者：
Nürnberg DJ
中科院分区：
文献类型：
--
作者：
Antonaru LA;Cardona T;Larkum AWD;Nürnberg DJ
有些蓝细菌利用可见光谱以外的光进行光合作用。远红光（FRL）区是通过一个复杂的驯化过程，涉及到新的藻胆体和含叶绿素f的光系统的形成。从单细胞到分支丝状形式的各种蓝藻都表现出这种反应。这些生物体已经从阴影环境中分离出来，如微生物垫，土壤，岩石和基质。然而，含有叶绿素f的物种在自然界中的全面传播仍然是未知的。目前，发现新的蓝藻叶绿素需要在选择性远红光下长时间的孵育。我们已经使用标记基因来检测环境样品和宏基因组数据中的叶绿素f生物体。该标记apcE 2编码与FRL-光合作用相关的藻胆体接头。通过聚焦于序列内的远红基序，简并PCR和BLAST搜索可以有效地区分正常的叶绿素a相关的apcE。即使是短恢复序列携带足够的信息系统发育的位置。在来自地球仪不同环境的宏基因组数据集中发现了叶绿素f光合作用的标记，包括蓝藻共生体、高盐湖泊、珊瑚和北极/南极地区。这些额外的信息使更高的系统发育分辨率支持的假设，垂直下降，而不是水平基因转移，主要是负责这种表型的分布。
Some cyanobacteria use light outside the visible spectrum for oxygenic photosynthesis. The far-red light (FRL) region is made accessible through a complex acclimation process that involves the formation of new phycobilisomes and photosystems containing chlorophyll f. Diverse cyanobacteria ranging from unicellular to branched-filamentous forms show this response. These organisms have been isolated from shaded environments such as microbial mats, soil, rock, and stromatolites. However, the full spread of chlorophyll f-containing species in nature is still unknown. Currently, discovering new chlorophyll f cyanobacteria involves lengthy incubation times under selective far-red light. We have used a marker gene to detect chlorophyll f organisms in environmental samples and metagenomic data. This marker, apcE2, encodes a phycobilisome linker associated with FRL-photosynthesis. By focusing on a far-red motif within the sequence, degenerate PCR and BLAST searches can effectively discriminate against the normal chlorophyll a-associated apcE. Even short recovered sequences carry enough information for phylogenetic placement. Markers of chlorophyll f photosynthesis were found in metagenomic datasets from diverse environments around the globe, including cyanobacterial symbionts, hypersaline lakes, corals, and the Arctic/Antarctic regions. This additional information enabled higher phylogenetic resolution supporting the hypothesis that vertical descent, as opposed to horizontal gene transfer, is largely responsible for this phenotype’s distribution.
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影响因子：
3.7
作者：
Cardona T;Sánchez-Baracaldo P;Rutherford AW;Larkum AW
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