Diauxic shift-dependent relocalization of decapping activators Dhh1 and Pat1 to polysomal complexes.
Diauxic shift-dependent relocalization of decapping activators Dhh1 and Pat1 to polysomal complexes.
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DOI：
10.1093/nar/gkr474
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发表时间：
2011-09-01
影响因子：
14.9
通讯作者：
McCarthy JE
中科院分区：
文献类型：
--
作者：
Drummond SP;Hildyard J;Firczuk H;Reamtong O;Li N;Kannambath S;Claydon AJ;Beynon RJ;Eyers CE;McCarthy JE
Dhh 1和Pat 1是酵母中的mRNA去帽激活子/翻译抑制子，被认为在mRNA从翻译到降解的转变中起关键作用。然而，很少有人知道这些蛋白质和翻译机制之间的物理和功能关系。我们描述了一个以前未知的类型的二次移位依赖调制的细胞内位置的Dhh 1和Pat 1。与P小体的形成一样，这种现象改变了参与翻译和mRNA降解的组分之间的空间关系。我们报告显着的空间分离Dhh 1和Pat 1的核糖体在指数增长的细胞。此外，生化分析表明，这些蛋白质被排除在多聚体复合物在指数增长的细胞，表明它们可能不与翻译机器的活跃状态。相反，在二次生长移位条件下，Dhh 1和帕特1被发现与多聚体复合物共定位。这项工作表明，Dhh 1和Pat 1的功能是由涉及eIF 4A的重新定位机制调制的。下拉实验表明，Dhh 1和Pat 1的细胞内结合伙伴的变化，细胞进行二次生长转变。这揭示了一个新的层面之间的关系，翻译活动和相互作用的mRNA，翻译机器和decapping激活蛋白。
Dhh1 and Pat1 in yeast are mRNA decapping activators/translational repressors thought to play key roles in the transition of mRNAs from translation to degradation. However, little is known about the physical and functional relationships between these proteins and the translation machinery. We describe a previously unknown type of diauxic shift-dependent modulation of the intracellular locations of Dhh1 and Pat1. Like the formation of P bodies, this phenomenon changes the spatial relationship between components involved in translation and mRNA degradation. We report significant spatial separation of Dhh1 and Pat1 from ribosomes in exponentially growing cells. Moreover, biochemical analyses reveal that these proteins are excluded from polysomal complexes in exponentially growing cells, indicating that they may not be associated with active states of the translation machinery. In contrast, under diauxic growth shift conditions, Dhh1 and Pat1 are found to co-localize with polysomal complexes. This work suggests that Dhh1 and Pat1 functions are modulated by a re-localization mechanism that involves eIF4A. Pull-down experiments reveal that the intracellular binding partners of Dhh1 and Pat1 change as cells undergo the diauxic growth shift. This reveals a new dimension to the relationship between translation activity and interactions between mRNA, the translation machinery and decapping activator proteins.
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DOI：
10.1038/nrd1853
发表时间：
2005-10
期刊：
Nature reviews. Drug discovery
影响因子：
--
作者：
Kwong AD;Rao BG;Jeang KT
通讯作者：
Jeang KT
影响因子：
4.8
作者：
Piccininni, S;Varaklioti, A;McCarthy, JEG
通讯作者：
McCarthy, JEG
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
3.6
作者：
Berthelot, K;Muldoon, M;McCarthy, JEG
通讯作者：
McCarthy, JEG
影响因子：
4.8
作者：
Koloteva, N;Muller, PP;McCarthy, JEG
通讯作者：
McCarthy, JEG