LncRNA LOC100129620 promotes osteosarcoma progression through regulating CDK6 expression, tumor angiogenesis, and macrophage polarization.
LncRNA LOC100129620 promotes osteosarcoma progression through regulating CDK6 expression, tumor angiogenesis, and macrophage polarization.
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DOI：
10.18632/aging.203042
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发表时间：
2021-05-18
期刊：
影响因子：
--
通讯作者：
Shen Y
中科院分区：
文献类型：
--
作者：
Chen Y;Tang G;Qian H;Chen J;Cheng B;Zhou C;Shen Y
骨肉瘤是儿童和青少年的一种死亡率很高的恶性肿瘤。骨肉瘤转移的机制目前尚不清楚。长链非编码RNA（lncRNA）的异常表达在肿瘤转移中起重要作用。我们运用生物信息学方法分析骨肉瘤原位癌和骨肉瘤肺转移癌基因表达的差异。CCK-8法检测lncRNA LOC 100129620对骨肉瘤细胞增殖的影响。Transwell法检测LOC 100129620对骨肉瘤细胞侵袭能力的影响。使用RNA pull-down和荧光素酶报告基因试验检查了LOC 100129620对miR-335- 3 p的调节作用。实时荧光定量PCR检测LOC 100129620对巨噬细胞极化的影响。结果表明，LOC 100129620能促进骨肉瘤细胞的增殖和迁移。LOC 100129620在体内可促进骨肉瘤的增殖。LOC 100129620可以与miR-335- 3 p结合并调节其功能。miR-335- 3 p介导LOC 100129620对CDK 6的调节作用。LOC 100129620促进血管形成和巨噬细胞极化。LOC 100129620/miR-335- 3 p/CDK 6信号通路通过调节骨肉瘤细胞增殖、血管生成和巨噬细胞极化促进骨肉瘤转移。
Osteosarcoma is a malignant tumor with high mortality in children and adolescents. The mechanism of osteosarcoma metastasis is currently unclear. Abnormal expression of long non-coding RNA (lncRNA) plays an important role in tumor metastasis. We used bioinformatics to analyze the differences in gene expression between osteosarcoma in situ and osteosarcoma lung metastases. CCK-8 was used to detect the effect of lncRNA LOC100129620 on the proliferation of osteosarcoma cells. The effect of LOC100129620 on the invasion of osteosarcoma cells was assessed by Transwell assay. The regulatory effect of LOC100129620 on miR-335-3p was examined using RNA pull-down and luciferase reporter gene assays. The effect of LOC100129620 on the polarization of macrophages was detected by quantitative real-time fluorescent PCR. The results show that LOC100129620 can promote the proliferation and migration of osteosarcoma cells. LOC100129620 can promote the proliferation of osteosarcoma in vivo. LOC100129620 can bind to miR-335-3p and regulate its function. MiR-335-3p mediates the regulatory effects of LOC100129620 on CDK6. LOC100129620 promotes the formation of blood vessels and the polarization of macrophages. The LOC100129620/miR-335-3p/CDK6 signaling pathway promotes the metastasis of osteosarcoma by regulating the proliferation of osteosarcoma cells, angiogenesis, and macrophage polarization.
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DOI：
10.1080/21691401.2019.1648285
发表时间：
2019-01-01
影响因子：
5.8
作者：
Chen, Xinxin;Zhang, Chengyong;Wang, Xiao
通讯作者：
Wang, Xiao
长链非编码 RNA 在骨稳态中的新作用及其在骨相关疾病中的潜在应用
影响因子：
3.1
作者：
Song, Wei;Xie, Jiahui;Xiao, Yu
通讯作者：
Xiao, Yu
影响因子：
5.2
作者：
Fu, Dong;Lu, Chunwen;Zhu, Xiaodong
通讯作者：
Zhu, Xiaodong
影响因子：
5.2
作者：
Wang, Xuesong;Peng, Lei;Sun, Ruifu
通讯作者：
Sun, Ruifu
影响因子：
11.4
作者：
Zhang, Jiahao;Li, Huachao;Liu, Bing
通讯作者：
Liu, Bing