Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture.
Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture.
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DOI：
10.1038/s41598-017-18630-1
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发表时间：
2018-01-10
影响因子：
4.6
通讯作者：
Bernstein HC
中科院分区：
文献类型：
--
作者：
Khan N;Maezato Y;McClure RS;Brislawn CJ;Mobberley JM;Isern N;Chrisler WB;Markillie LM;Barney BM;Song HS;Nelson WC;Bernstein HC
不同的微生物物种在特定环境中是共存还是竞争的基本问题取决于环境、组成和环境限制。模型微生物系统可以产生一些与这个问题有关的一般原理。在这项研究中，我们采用了一种自然发生的共培养物组成的异养细菌，盐单胞菌属HL-48和海蛞蝓属HL-58，问两个基本的科学问题：1）如何表型的两个自然共存的物种响应伙伴关系相比，无菌生长？以及2）这些物种的生长和分子表型如何随着竞争和竞争相互作用而改变？我们假设-并证实-在葡萄糖作为唯一碳源下的共培养将导致竞争性相互作用。类似地，当葡萄糖与木糖交换时，相互作用变得不稳定，因为MarinocytoHL-58由来自盐单胞菌HL-48的代谢物支持。每个物种都通过改变其生长和分子表型来响应伙伴关系，这通过批量生长动力学和全球转录组学来测定。这些表型反应取决于养分的可利用性，因此环境最终控制了它们如何相互反应。这个简化的模型社区揭示了微生物的相互作用是特定于环境的，不同的环境条件决定了物种间的伙伴关系将如何展开。
The fundamental question of whether different microbial species will co-exist or compete in a given environment depends on context, composition and environmental constraints. Model microbial systems can yield some general principles related to this question. In this study we employed a naturally occurring co-culture composed of heterotrophic bacteria, Halomonas sp. HL-48 and Marinobacter sp. HL-58, to ask two fundamental scientific questions: 1) how do the phenotypes of two naturally co-existing species respond to partnership as compared to axenic growth? and 2) how do growth and molecular phenotypes of these species change with respect to competitive and commensal interactions? We hypothesized – and confirmed – that co-cultivation under glucose as the sole carbon source would result in competitive interactions. Similarly, when glucose was swapped with xylose, the interactions became commensal because Marinobacter HL-58 was supported by metabolites derived from Halomonas HL-48. Each species responded to partnership by changing both its growth and molecular phenotype as assayed via batch growth kinetics and global transcriptomics. These phenotypic responses depended on nutrient availability and so the environment ultimately controlled how they responded to each other. This simplified model community revealed that microbial interactions are context-specific and different environmental conditions dictate how interspecies partnerships will unfold.
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影响因子：
--
作者：
Schroeder A;Mueller O;Stocker S;Salowsky R;Leiber M;Gassmann M;Lightfoot S;Menzel W;Granzow M;Ragg T
通讯作者：
Ragg T
影响因子：
4.4
作者：
Christensen, BB;Haagensen, JAJ;Molin, S
通讯作者：
Molin, S
影响因子：
11
作者：
Beliaev, Alexander S.;Romine, Margie F.;Konopka, Allan
通讯作者：
Konopka, Allan
DOI：
10.1007/bf00871225
发表时间：
1993-01-01
影响因子：
2.6
作者：
GOTTSCHAL, JC
通讯作者：
GOTTSCHAL, JC
影响因子：
4.1
作者：
Bernstein, Hans C.;Paulson, Steven D.;Carlson, Ross P.
通讯作者：
Carlson, Ross P.