The evolutionary path of chemosensory and flagellar macromolecular machines in Campylobacterota.
The evolutionary path of chemosensory and flagellar macromolecular machines in Campylobacterota.
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弯曲杆菌门化学感应和鞭毛大分子机器的进化路径
DOI：
10.1371/journal.pgen.1010316
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发表时间：
2022-07
期刊：
影响因子：
4.5
通讯作者：
中科院分区：
文献类型：
--
作者：
大分子复合物的进化是一个基本的生物学问题，它关系到生命的起源，也指导着合成生物学的实践。化学感受系统是在细菌中非常早期进化的复杂结构之一，并且在现代物种的组成和阵列结构方面显示出巨大的多样性和复杂性。然而，化学感受系统的多样性和复杂性是如何进化的仍然不清楚。在这里，使用一个强大的“生态evo”框架的弯曲杆菌门，我们调查的化学感受系统和它的重要信号输出之一，鞭毛机械的共同进化。我们的分析表明，大量的鞭毛基因的改变将导致开关的主要化学感受类从一个到另一个，或导致两个类的杂交。出乎意料的是，我们发现空肠弯曲杆菌和幽门螺杆菌的高扭矩产生鞭毛马达结构可能是在弯曲杆菌门的最后共同祖先中进化的。后来经历明显鞭毛改变的谱系失去了复杂支架结构的一些关键成分，从而衍生出比它们的祖先更简单的结构。总体而言，本研究揭示了化学感受系统和鞭毛系统的协同进化路径，并强调鞭毛结构复杂性的进化需要基于解析的系统发育框架在细菌领域进行更多研究，而不对进化方向进行假设。化学感受系统是细菌中最复杂的信号转导系统，不同种类的细菌在组成和结构上存在很大差异。其重要的信号输出之一是由螺旋桨驱动的鞭毛运动，螺旋桨由数十种蛋白质组成，并且在不同物种中围绕核心运动结构显示出相当大的变化和复杂性。化学感受系统和鞭毛的进化都是重要的生物学问题，但至今仍不清楚。本论文对细菌化学感受系统和鞭毛结构的进化路径进行了详细的研究，为它们的协同进化提供了分子证据。我们的研究提供了一个范例，研究的基础上强大的细菌共生和基因组背景下的共同进化的系统/组件的大分子复合物的进化。
The evolution of macromolecular complex is a fundamental biological question, which is related to the origin of life and also guides our practice in synthetic biology. The chemosensory system is one of the complex structures that evolved very early in bacteria and displays enormous diversity and complexity in terms of composition and array structure in modern species. However, how the diversity and complexity of the chemosensory system evolved remains unclear. Here, using the Campylobacterota phylum with a robust “eco-evo” framework, we investigated the co-evolution of the chemosensory system and one of its important signaling outputs, flagellar machinery. Our analyses show that substantial flagellar gene alterations will lead to switch of its primary chemosensory class from one to another, or result in a hybrid of two classes. Unexpectedly, we discovered that the high-torque generating flagellar motor structure of Campylobacter jejuni and Helicobacter pylori likely evolved in the last common ancestor of the Campylobacterota phylum. Later lineages that experienced significant flagellar alterations lost some key components of complex scaffolding structures, thus derived simpler structures than their ancestor. Overall, this study revealed the co-evolutionary path of the chemosensory system and flagellar system, and highlights that the evolution of flagellar structural complexity requires more investigation in the Bacteria domain based on a resolved phylogenetic framework, with no assumptions on the evolutionary direction. Chemosensory system is the most complicated signal transduction system in bacteria with great diversity in both composition and structural organization across species. One of its important signaling output is flagellar motility driven by a propeller, which is made of dozens of proteins and shows considerable variation and complexity surrounding the core motor structure in different species. The evolution of both chemosensory system and flagellum are important biological questions but remain obscure. Here, we carefully examined the evolutionary paths of chemosensory system and flagellar structure in a bacterial phylum, providing detailed molecular evidences for their co-evolution. Our study provides a paradigm to study the evolution of macromolecular complexes based on robust bacterial phylogeny and co-evolved systems/components in genome context.
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影响因子：
6.4
作者：
Burnham, Peter M.;Kolar, William P.;Hendrixson, David R.
通讯作者：
Hendrixson, David R.
DOI：
10.1073/pnas.1902521116
发表时间：
2019-07-30
影响因子：
11.1
作者：
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通讯作者：
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DOI：
10.7717/peerj-cs.251
发表时间：
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期刊：
PeerJ. Computer science
影响因子：
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作者：
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通讯作者：
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DOI：
10.1073/pnas.1604693113
发表时间：
2016-09-13
影响因子：
11.1
作者：
Briegel, Ariane;Ortega, Davi R.;Jensen, Grant J.
通讯作者：
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