Mouse spermatozoa with higher fertilization rates have thinner nuclei.
Mouse spermatozoa with higher fertilization rates have thinner nuclei.
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作者：
Mashiko D;Ikawa M;Fujimoto K
尽管形态正常的精子被认为具有相同的受精能力，但最近的数据表明，即使是正常的精子，其头部形状也有相当大的差异，这与受精能力的差异有关。然而，良好精子形态的适当定量指标仍未确定。因此，为了识别这样的指标，我们使用椭圆傅立叶描述子和主成分分析相结合的多变量定量分析方法对正常小鼠精子的核轮廓进行了比较。这些精子来自不同的品系和采集点，已被证明与不同的受精能力有关。我们发现，B6D2F1(BDF1)株的精子头部比C57BL/6N(B6N)株的精子薄5.7%，BDF1株的受精率较高，而C57BL/6N(B6N)株的受精率较低。此外，卵黄周隙中穿透透明带的精子头部比射精前从附睾处分离的精子头部始终薄5.4%。代表精子头部厚度的纵横比唯一地区分了这些精子群体，证实了其作为形态指标的有效性。由于长宽比也被证明是人类精子的特征，这一独特的形态测量指标可能适用于多个患者之间的正常精子比较，这将极大地促进和提高现有的生殖技术。
Although spermatozoa with normal morphology are assumed to have uniform fertilization ability, recent data show that even normal spermatozoa have considerable variation in their head shape which is associated with differences in fertilization ability. Appropriate quantitative indicators for good sperm morphology, however, remain unidentified. Therefore, in an effort to identify such an indicator, we compared the nuclear contour of normal mouse spermatozoa by quantitative multivariate analysis using elliptic Fourier descriptors combined with principal component analysis. The spermatozoa were obtained from different strains and collection sites which have been shown to be associated with different fertilization abilities. We found that the head was 5.7% thinner in spermatozoa from the B6D2F1 (BDF1) strain, known to have a higher fertilization rate, than in those from the C57BL/6N (B6N) strain, which has a lower fertilization rate. Moreover, zona-penetrated spermatozoa in the perivitelline space consistently had 5.4% thinner heads than those isolated from the epididymis before ejaculation. The aspect ratio, which represents the sperm head thinness, uniquely distinguished these sperm populations, confirming its validity as a morphological indicator. Because aspect ratio has also been shown to characterize human spermatozoa, this unique morphometric indicator might be applicable to compare normal spermatozoa among multiple patients, which will greatly facilitate and enhance current reproductive technologies.
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DOI：
10.1530/jrf.0.0390297
发表时间：
1974-01-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
COHEN, J;MCNAUGHTON, DC
通讯作者：
MCNAUGHTON, DC
影响因子：
3.1
作者：
Iwata, H;Ukai, Y
通讯作者：
Ukai, Y
影响因子：
3.6
作者：
Nakanishi, T;Isotani, A;Okabe, M
通讯作者：
Okabe, M
影响因子：
--
作者：
JOUANNET, P;DUCOT, B;SPIRA, A
通讯作者：
SPIRA, A
影响因子：
6.1
作者：
MENKVELD, R;STANDER, FSH;VANZYL, JA
通讯作者：
VANZYL, JA