Ly-49D and Ly-49H associate with mouse DAP12 and form activating receptors.
Ly-49D and Ly-49H associate with mouse DAP12 and form activating receptors.
复制标题
Ly-49D 和 Ly-49H 与小鼠 DAP12 结合并形成激活受体。
作者：
Kathleen M. Smith;Jun Wu;A. Bakker;J. Phillips;L. Lanier
Ly-49受体家族的几个成员抑制NK细胞介导的表达适当MHC I类分子的靶分子的裂解。Ly-49D和Ly-49H是两个胞质结构域缺乏免疫受体酪氨酸抑制基序(ITIM)的Ly-49分子，它们与小鼠DAP12相关，DAP12是一个具有免疫受体酪氨酸激活基序(ITAM)的分子。将Ly-49D或Ly-49H与DAP12共转染可诱导Ly-49和DAP12的表面表达。Ly-49/DAP12复合体免疫共沉淀，证实DAP12与细胞膜上的Ly-49D或Ly-49H发生物理结合。用识别Ly-49D或Ly-49H的抗体刺激转染体，通过诱导多种细胞底物的酪氨酸磷酸化来评估细胞激活。
Several members of the Ly-49 receptor family inhibit NK cell-mediated lysis of targets expressing appropriate MHC class I molecules. Ly-49D and Ly-49H, two Ly-49 molecules that lack immunoreceptor tyrosine-based inhibitory motifs (ITIM) in their cytoplasmic domains, associate with mouse DAP12, a molecule that possesses an immunoreceptor tyrosine-based activation motif (ITAM). Cotransfection of either Ly-49D or Ly-49H with DAP12 induces surface expression of both Ly-49 and DAP12. The Ly-49/DAP12 complex was coimmunoprecipitated from the transfected cells, demonstrating a physical association of DAP12 with Ly-49D or Ly-49H in the plasma membrane. Stimulation of transfectants with Abs recognizing either Ly-49D or Ly-49H results in cellular activation, as assessed by induction of tyrosine phosphorylation of multiple cellular substrates.
逆转录病毒介导的表达克隆的应用。
DOI：
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发表时间：
1996
期刊：
Experimental hematology.
影响因子：
--
作者：
Onishi,M;Kinoshita,S;Morikawa,Y;Shibuya,A;Phillips,J;Lanier,LL;Gorman,DM;Nolan,GP;Miyajima,A;Kitamura,T
通讯作者：
Kitamura,T
由 IL-2 激活的 NK 细胞表达的 Ly-49 多基因家族。
DOI：
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发表时间：
1994
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Smith,HR;Karlhofer,FM;Yokoyama,WM
通讯作者：
Yokoyama,WM
影响因子：
32.4
作者：
Valiante, NM;Uhrberg, M;Parham, P
通讯作者：
Parham, P