IL-22 is expressed by the invasive trophoblast of the equine (Equus caballus) chorionic girdle.
IL-22 is expressed by the invasive trophoblast of the equine (Equus caballus) chorionic girdle.
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DOI：
10.4049/jimmunol.1103509
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发表时间：
2012-05-01
期刊：
影响因子：
--
通讯作者：
Antczak DF
中科院分区：
文献类型：
--
作者：
Brosnahan MM;Miller DC;Adams M;Antczak DF
马胎盘的浸润性滋养层细胞迁移到子宫内膜形成子宫内膜杯，在正常妊娠的40 - 120天之间，滋养层细胞密集堆积，产生马绒毛膜促性腺激素。滋养层细胞侵入子宫内膜同时逃避母体免疫破坏的机制尚不清楚。对妊娠第34天获得的胎盘组织进行的基因表达微阵列分析显示，相对于非侵入性绒毛膜，绒毛膜带中编码细胞因子IL-22的mRNA上调>900倍。用定量RT-PCR检测绒毛带中IL-22的高表达。另外的定量RT-PCR分析显示，从第32天到第35天，绒毛膜带中IL-22 mRNA表达显著增加，而在其他孕体组织中没有IL-22表达。生物信息学分析和cDNA测序证实了马IL-22的预测长度，其携带人和小鼠IL-22基因中不存在的39个延伸，但存在于牛和猪中。我们在绒毛膜带中发现IL-22是一个新的发现，因为这种细胞因子以前只在免疫细胞中报道过。IL-22具有免疫调节功能，主要作用于上皮细胞。IL-22 R1 mRNA在妊娠子宫内膜中的表达水平与其他马上皮细胞相似。基于这些发现，我们推测绒毛膜带产生的IL-22细胞因子与子宫内膜上的IL-22 R1结合，通过调节子宫内膜对滋养层侵袭的反应，作为胎儿-母体通信的机制。
The invasive trophoblast cells of the equine placenta migrate into the endometrium to form endometrial cups, dense accumulations of trophoblast cells that produce equine chorionic gonadotropin between days 40 and 120 of normal pregnancy. The mechanisms by which the trophoblast cells invade the endometrium while evading maternal immune destruction are poorly defined. A gene expression microarray analysis performed on placental tissues obtained at day 34 of gestation revealed a >900-fold upregulation of mRNA encoding the cytokine IL-22 in chorionic girdle relative to noninvasive chorion. Quantitative RT-PCR assays were used to verify high expression of IL-22 in chorionic girdle. Additional quantitative RT-PCR analysis showed a striking increase in IL-22 mRNA expression in chorionic girdle from days 32 to 35 and an absence of IL-22 expression in other conceptus tissues. Bio-informatic analysis and cDNA sequencing confirmed the predicted length of horse IL-22, which carries a 39 extension absent in IL-22 genes of humans and mice, but present in the cow and pig. Our discovery of IL-22 in the chorionic girdle is a novel finding, as this cytokine has been previously reported in immune cells only. IL-22 has immunoregulatory functions, with primary action on epithelial cells. mRNA of IL-22R1 was detected in pregnant endometrium at levels similar to other equine epithelia. Based upon these findings, we hypothesize that IL-22 cytokine produced by the chorionic girdle binds IL-22R1 on endometrium, serving as a mechanism of fetal-maternal communication by modulating endometrial responses to trophoblast invasion.
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影响因子：
--
作者：
ALLEN, WR;HAMILTON, DW;MOOR, RM
通讯作者：
MOOR, RM
影响因子：
4.1
作者：
Bousfield, GR;Butnev, VY;Moore, WT
通讯作者：
Moore, WT
影响因子：
4.4
作者：
Faria, Miriam R.;Hoshida, Mara S.;Bevilacqua, Estela
通讯作者：
Bevilacqua, Estela
DOI：
10.1530/jrf.0.1170143
发表时间：
1999-09-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
Gerstenberg, C;Allen, WR;Stewart, E
通讯作者：
Stewart, E
影响因子：
4.4
作者：
Kotenko, SV;Izotova, LS;Pestka, S
通讯作者：
Pestka, S