NECAB1 and NECAB2 are Prevalent Calcium-Binding Proteins of CB1/CCK-Positive GABAergic Interneurons.
NECAB1 and NECAB2 are Prevalent Calcium-Binding Proteins of CB1/CCK-Positive GABAergic Interneurons.
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NECAB 1和NECAB 2是CB 1/CCK阳性GABA能中间神经元的普遍钙结合蛋白。
DOI：
10.1093/cercor/bhaa326
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发表时间：
2021-02-05
期刊：
影响因子：
--
通讯作者：
Katona I
中科院分区：
文献类型：
--
作者：
Miczán V;Kelemen K;Glavinics JR;László ZI;Barti B;Kenesei K;Kisfali M;Katona I
“Ca 2+信号工具包”的分子库支持不同神经元类型中Ca 2+依赖性过程的特定动力学要求。一个众所周知的例子是钙结合蛋白的独特表达模式，如小清蛋白、钙结合蛋白和钙视网膜蛋白。这些胞质Ca 2+缓冲液以细胞类型特异性方式控制突触前和体树突过程，并且几十年来一直被用作GABA能中间神经元类型的神经化学标记物。令人惊讶的是，迄今为止，在CB 1大麻素受体/胆囊收缩素（CB 1/CCK）阳性中间神经元中没有发现典型的钙结合蛋白，这些中间神经元代表了皮层回路中大量的GABA能细胞。由于CB 1/CCK阳性中间神经元显示不同的突触前和体树突钙瞬变与其他中间神经元相比，我们测试的假设，他们表达替代钙结合蛋白。通过在小鼠单细胞RNA-seq数据库中进行计算机数据挖掘，我们在CB 1/CCK阳性中间神经元中分别鉴定了编码N-末端EF-手型钙结合蛋白1和2的Necab 1和Necab 2基因的高表达。荧光原位杂交和免疫染色显示细胞类型特异性分布的NECAB 1和NECAB 2整个同皮质，海马结构，基底外侧杏仁核复合体。结合膜片钳电生理学，共聚焦，和STORM超分辨率显微镜揭示了亚细胞纳米级的差异，表明两种钙结合蛋白之间的劳动力的功能分工。这些发现突出了NECAB 1和NECAB 2作为CB 1/CCK阳性中间神经元中的主要钙结合蛋白。
The molecular repertoire of the “Ca2+-signaling toolkit” supports the specific kinetic requirements of Ca2+-dependent processes in different neuronal types. A well-known example is the unique expression pattern of calcium-binding proteins, such as parvalbumin, calbindin, and calretinin. These cytosolic Ca2+-buffers control presynaptic and somatodendritic processes in a cell-type-specific manner and have been used as neurochemical markers of GABAergic interneuron types for decades. Surprisingly, to date no typifying calcium-binding proteins have been found in CB1 cannabinoid receptor/cholecystokinin (CB1/CCK)-positive interneurons that represent a large population of GABAergic cells in cortical circuits. Because CB1/CCK-positive interneurons display disparate presynaptic and somatodendritic Ca2+-transients compared with other interneurons, we tested the hypothesis that they express alternative calcium-binding proteins. By in silico data mining in mouse single-cell RNA-seq databases, we identified high expression of Necab1 and Necab2 genes encoding N-terminal EF-hand calcium-binding proteins 1 and 2, respectively, in CB1/CCK-positive interneurons. Fluorescent in situ hybridization and immunostaining revealed cell-type-specific distribution of NECAB1 and NECAB2 throughout the isocortex, hippocampal formation, and basolateral amygdala complex. Combination of patch-clamp electrophysiology, confocal, and STORM super-resolution microscopy uncovered subcellular nanoscale differences indicating functional division of labor between the two calcium-binding proteins. These findings highlight NECAB1 and NECAB2 as predominant calcium-binding proteins in CB1/CCK-positive interneurons.
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影响因子：
4.7
作者：
Canela, Laia;Fernandez-Duenas, Victor;Ciruela, Francisco
通讯作者：
Ciruela, Francisco
影响因子：
16.2
作者：
Blatow, M;Rozov, A;Monyer, H
通讯作者：
Monyer, H
影响因子：
5.8
作者：
Baddeley, A;Turner, R
通讯作者：
Turner, R
影响因子：
3.5
作者：
Cancla, Laia;Lujan, Rafael;Ciruela, Francisco
通讯作者：
Ciruela, Francisco
DOI：
10.1186/2192-2853-1-6
发表时间：
2012
期刊：
Optical nanoscopy
影响因子：
--
作者：
Babcock H;Sigal YM;Zhuang X
通讯作者：
Zhuang X