Pathway-specific feedforward circuits between thalamus and neocortex revealed by selective optical stimulation of axons.
Pathway-specific feedforward circuits between thalamus and neocortex revealed by selective optical stimulation of axons.
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DOI：
10.1016/j.neuron.2009.12.025
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发表时间：
2010-01-28
期刊：
影响因子：
16.2
通讯作者：
Connors, Barry W.
中科院分区：
文献类型：
--
作者：
Cruikshank, Scott J.;Urabe, Hayato;Nurmikko, Arto V.;Connors, Barry W.
丘脑皮质和皮质丘脑通路介导丘脑和新皮质之间的双向通讯。这些途径相互交织，使他们的研究充满挑战。在这里，我们使用慢病毒在丘脑皮质或皮质丘脑投射细胞中表达通道视紫红质-2 (ChR2)，这是一种光敏阳离子通道。感染仅发生在局部，但传出轴突及其末端强烈表达 ChR2，从而允许选择性光学激活每个通路。激光刺激表达 ChR2 的丘脑皮质轴突/末端在皮质兴奋性细胞和快速尖峰 (FS) 抑制性中间神经元中引起强烈的突触反应，但在含有生长抑素的中间神经元中仅产生微弱的反应。强烈的 FS 细胞激活导致所有皮质神经元类型（包括 FS 细胞）的前馈抑制。皮质丘脑刺激会兴奋丘脑中继细胞和丘脑网状核（TRN）的抑制性神经元。 TRN 激活会触发中继细胞的抑制，但不会触发 TRN 神经元的抑制。因此，丘脑皮质和皮质丘脑处理之间的主要区别在于前馈抑制神经元本身受前馈抑制的程度。
Thalamocortical and corticothalamic pathways mediate bidirectional communication between the thalamus and neocortex. These pathways are entwined, making their study challenging. Here we used lentiviruses to express channelrhodopsin-2 (ChR2), a light-sensitive cation channel, in either thalamocortical or corticothalamic projection cells. Infection occurred only locally but efferent axons and their terminals expressed ChR2 strongly, allowing selective optical activation of each pathway. Laser stimulation of ChR2-expressing thalamocortical axons/terminals evoked robust synaptic responses in cortical excitatory cells and fast-spiking (FS) inhibitory interneurons, but only weak responses in somatostatin-containing interneurons. Strong FS cell activation led to feedforward inhibition in all cortical neuron types, including FS cells. Corticothalamic stimulation excited thalamic relay cells and inhibitory neurons of the thalamic reticular nucleus (TRN). TRN activation triggered inhibition in relay cells but not in TRN neurons. Thus, a major difference between thalamocortical and corticothalamic processing was the extent to which feedforward inhibitory neurons were themselves engaged by feedforward inhibition.
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影响因子：
25
作者：
Cruikshank, Scott J.;Lewis, Timothy J.;Connors, Barry W.
通讯作者：
Connors, Barry W.
影响因子：
2.5
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影响因子：
25
作者：
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影响因子：
3.3
作者：
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通讯作者：
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影响因子：
2.5
作者：
Beierlein, M;Gibson, JR;Connors, BW
通讯作者：
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