Effects of different oxygen concentrations on the proliferation, survival, migration, and osteogenic differentiation of MC3T3-E1 cells
Effects of different oxygen concentrations on the proliferation, survival, migration, and osteogenic differentiation of MC3T3-E1 cells
复制标题
不同氧浓度对MC3T3-E1细胞增殖、存活、迁移和成骨分化的影响
DOI：
10.1080/03008207.2018.1487413
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发表时间：
2019-04
影响因子：
2.9
通讯作者：
Lixin Zhu
中科院分区：
文献类型：
--
作者：
Haixin Liu;Minsheng Yang;Guofeng Wu;Lianjun Yang;Yanlin Cao;Chun Liu;Zhiwen Tan;Yanglei Jin;Jiasong Guo;Lixin Zhu
ABSTRACT In physiological and pathological environments, the concentration of oxygen around osteoblasts varies widely. No studies have systematically evaluated the effects of different oxygen concentrations on the proliferation, survival, migration, and osteogenic differentiation of osteoblasts. In this study, we cultured the osteoblast precursor cell line MC3T3-E1 in small individual chambers with oxygen concentrations of 1%, 3%, 6%, 9%, and 21%. Cell proliferation was evaluated by the proliferation index test and EdU staining. To test cell survival, a live/dead assay was performed. A tablet scratch assay was performed to detect the migratory ability of the cells. Bone nodule formation experiments and immunofluorescence and Western blotting analyses of osteogenic-related proteins were performed to assess the osteogenic differentiation of the cells. We found that the proliferation and osteogenic differentiation ability of MC3T3-E1 cells in different oxygen concentrations were both approximately bell-shaped curves and that the optimal oxygen concentrations were approximately 6% and 9%, respectively. The live/dead assay showed that the survival of MC3T3-E1 cells in different oxygen concentrations was affected by the amount of serum. The tablet scratch experiment showed that there was greater cell migration with oxygen concentrations of 1%, 3%, and 21% than with oxygen concentrations of 6% and 9%. Our results have significant reference value for the intervention of the pathological processes involving osteoblasts, such as fracture, osteoporosis, and some vascular diseases. These results also have an important guiding role for the new scientific idea that osteoblasts can function as treatment cells to repair bone defects.
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影响因子：
3.1
作者：
Bruderer, M.;Richards, R. G.;Stoddart, M. J.
通讯作者：
Stoddart, M. J.
影响因子：
4.1
作者：
Re-Wen Wu;Tzu-Ping Lin;J. Ko;Da-Wei Yeh;Ming-Wen Chen;Huei-Ching Ke;Shin-Long Wu;Feng-Sheng Wang
通讯作者：
Re-Wen Wu;Tzu-Ping Lin;J. Ko;Da-Wei Yeh;Ming-Wen Chen;Huei-Ching Ke;Shin-Long Wu;Feng-Sheng Wang
影响因子：
2.1
作者：
Lechler, Philipp;Klein, Silvan M.;Grifka, Joachim
通讯作者：
Grifka, Joachim
影响因子：
6.2
作者：
Kato, Y;Boskey, K;Bonewald, LF
通讯作者：
Bonewald, LF
影响因子：
3.4
作者：
Lichao Wang;Buling Wu;Yu Zhang;Zhihui Tian
通讯作者：
Lichao Wang;Buling Wu;Yu Zhang;Zhihui Tian