Glutathione as a matrix for plasma desorption mass spectrometry of large peptides.
Glutathione as a matrix for plasma desorption mass spectrometry of large peptides.
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谷胱甘肽作为大肽等离子体解吸质谱分析的基质。
DOI：
10.1021/ac00298a008
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发表时间：
1986
影响因子：
7.4
通讯作者：
Robert J. Cotter
中科院分区：
文献类型：
--
作者：
M. Alai;P. Demirev;Catherine Fenselau;Robert J. Cotter
在含有谷胱甘肽的溶液中溶解和电喷雾的大肽的等离子体解吸质谱显示分子离子信号增加、基线噪声和峰宽度减少以及多电荷离子增加。造成这些影响的是还原形式的谷胱甘肽，而不是氧化形式。其他一些化学性质相似的基质表现出类似的效果，而另一些则没有。提出了基质的多种作用，包括先前报道的对溶液中蛋白质重折叠和聚集的影响，以及在解吸过程中降低样品/底物结合能的可能性。通过引入甘油 (1, 2)，作为快原子轰击技术的一个组成部分，强调了适当的化学/物理基质对于质谱分析中难处理化合物解吸的重要性。从一开始我们就知道，液体基质在高通量轰击下可提供强稳定的二次离子信号，这对于能够处理高质量范围的高性能双聚焦质谱仪来说是必需的 (3)。最近，还提出了通过溶剂脱落降低二次离子内能的可能性 (4, 5)。在甘油中添加酸或使用单硫代甘油可改善基质作为质子供体的作用 (3, 6)。其他基质，例如用于全氟链烷酸铯簇的四甘醇二甲醚 (6)，或硫醇混合物，例如用于肽的二硫苏糖醇和二硫赤藓糖醇 (7)，
The plasma desorption mass spectra of large peptides, dissolved and electrosprayed In solutions containing glutathione, show Increased molecular Ion signal, reduction of base-line noise and peak widths, and an Increase In multiply charged Ions. The reduced, rather than the oxidized, form of gluta-thione Is responsible for these effects. Some other chemically similar matrices show similar effects while others do not. Several roles for the matrix are suggested Including previously reported effects on protein refolding and aggregation In solution, as well as possibilities for lowering the sample/substrate binding energy during desorption.The importance of appropriate chemical/physical matrices for the desorption of intractable compounds in mass spec-trometry was underscored by the introduction of glycerol (1, 2), as an integral part of the fast atom bombardment technique. From the beginning it was understood that the liquid matrix providedstrong stable secondary ion signals under high-flux bombardment necessary for high-performance double-focusing mass spectrometers capable of high-mass ranges (3). More recently, the possibility for reducing the internal energy of secondary ions by solvent shedding has also been suggested (4, 5). The addition of acid to glycerol or the use of monothioglycerol improves the matrix’s role as a proton donor (3, 6). Other matrices, such as tetraglyme for cesium perfluoroalkonate clusters (6), or mixtures of thiols, such as dithiothreitol and dithioerythritol forpeptides (7), havebeen
热辅助快原子轰击：快原子轰击质谱分析优化的新方法。
影响因子：
7.4
作者：
Ackermann,BL;Watson,JT;Holland,JF
通讯作者：
Holland,JF